Relationship Between PRRX1,Circulating Tumor Cells,and Clinicopathological Parameter In Patients With Gastric Cancer

Objective: Paired related homoeobox 1(PRRX1)has been identified as a new epithelial-mesenchymal transition(EMT)inducer in gastric cancer and that PRRX1 upregulation is closely correlated with gastric cancer metastasis.In addition,Circulating tumor cells(CTCs)play an important role in the process of gastric cancer's distant metastasis.Our study aims to detect the expression of Prrx1 and CTCs in peripheral blood,and then analyze the relationship and clinical significance between PRRX1,CTCs and clinicpathological parameters in patients with gastric cancer.Method: Expressions of PRRX1 in a sample of 95 gastric carcinoma and adjacent nontumorous tissues were detected by immunohistochemistry.Then the integrated subtraction enrichment and immunostaining fluorescence in situ hybridization(SET-imFISH)platform were applied to detect and characterize CTCs in patients with gastric cancer.Finally,their correlations with clinicopathological parameter could be analyzed.Results: The positive rate of PRRX1 in gastric cancer was 56.84% and the rate was36.84% in adjacent normal gastric mucosa,which was confirmed to be significant by the statistical method(p=0.006).There was a close relationship between the PRRX1 of gastric cancer and the CTCs of peripheral blood specimens of cancer patients with the correlation coefficient 0.322(p=0.001).Both the expression of PRRX1 and the positive rate of CTCs did not significantly correlate with age,gender or histologic type(P>0.05)but significantly relate to tumor size,differentiated degree,depth of tumor invasion,lymph node metastasis,lymphatic vessel invasion,vascular invasion,metastasis status,and TNM stage(P<0.05).Conclusion: There was a positive correlation relationship between the PRRX1 of gastric cancer and the CTCs of peripheral blood specimens of cancer patients.The test of PRRX1 and CTCs may be uesd for evaluating the genesis,development of gastric cancer.They will be helpful to provide important reference for tumor's early diagnosis and forecast,especially for CTCs.And then both of them could provide important basis for gastric cancer clinical diagnosis and treatment...