Expression And Significance Of MAPK1,TNF-? And Myeloperoxidase In Peripheral Blood Of Patients With Preeclampsia

Objective:Through the detection of mapk1,TNF-a and serum myeloperoxidase levels in peripheral blood neutrophils of preeclampsia patients,and the analysis of their basic clinical data,to explore the correlation between the expression of mapk1,TNF-a and myeloperoxidase and preeclampsia.It is the basis for early clinical intervention to reduce the incidence of maternal and child complications and improve maternal and child outcomes.Methods:From October 2018 to December 2019,30 cases of pre eclampsia(Study Group)and 30 cases of normal pregnant women(control group)were admitted to the obstetric inpatient department of the second hospital of Shanxi Medical University,and the age range was 20-35 years old.At the same time,basic clinical data were collected.With the informed consent of the subjects and in accordance with the standards of medical ethics,8 ml peripheral blood of the patients was collected and put into anticoagulant tubes to extract neutrophils and separate serum.The expression of mapk1 and TNF-a in neutrophils was detected by Western blot,and the activity of myeloperoxidase in serum was detected by colorimetry.Spss26.0 software was used for statistical analysis.Results:1.Comparison of basic clinical data between two groups of pregnant women :compared with the control group,the systolic blood pressure,diastolic blood pressure,gestational age and neonatal weight increased significantly in the study group(P <0.01)[the systolic blood pressure were:(120.30 � 8.07),(166.30 � 14.79);Respectively:(76.87 � 5.78),(107.03 � 11.21);gestational weeks were:(39.6 � 1.1),(35.9 � 2.9)];age and BMI before pregnancy were not statistically significant(P >0.05).2.Western-blot test result comparison: There was no significant difference in MAPK1 expression in peripheral blood neutrophils(P > 0.05);the expression level of TNF-a in peripheral blood neutrophils was remarkably increased in the preeclampsia group,the difference was statistically significant[Gray values are:(0.34 � 0.05),(1.27� 0.10)](t = 10.360,P = 0.001).3.Colorimetric method: the serum myeloperoxidase activity in pregnant women's serum in preeclampsia group was significantly higher than that in normal pregnant women[Activities are:(37.78 � 10.81),(105.99 � 14.32)](t=3.58,P < 0.05).Conclusions:The expression level of TNF-a in peripheral blood neutrophils of patients with preeclampsia group was significantly increased,and serum myeloperoxidase activity was enhanced.There was no significant difference in the expression levels of MAPK1 in peripheral blood neutrophils.It is suggested that the increase of TNF-a and active MPO in neutrophils in peripheral blood of preeclampsia patients may be related to the pathogenesis of preeclampsia,and it is expected to be a predictive biomarker of preeclampsia.