A Study On Polarization Imaging Methods In Different Genotypes Of Breast Cancer

Breast cancer is the most prevalent cancer in female groups.Accurate and effective diagnostic methods is significant.The genotype of breast cancer is an important criterion for the treatment and prognosis of breast cancer.The current clinical detection can only be performed by immunohistochemical methods.However,its operation is complicated,while the cost is high and time-cosuming.The polarization imaging method can obtain rich microstructural information of biological tissue samples.Therefore,the polarization imaging method could be used to detect breast cancer cells and tissues and studied the relationship between polarization quantitative characterization parameters and the microstructure of different genotypes of breast cancer.The application of polarization imaging method in clinical diagnosis of breast cancer genotyping has guiding significance.In order to research the relationship between breast cancer genotyping and polarization parameters,this paper systematically studied the genotypes of breast cancer from both cell and tissue levels.Firstly,Mueller matrix microscopy was used to detect the backward Mueller matrix of four genotypes of breast cancer cell lines(Luminal A,Luminal B,Her-2 positive and Triple-negative)and use MMPD and MMT to obtain different polarization parameters.Among them,the MMT parameter t2 can provide effective information on the microscopic structure at the cell boundary,and there are obvious differences in the polarization parameters between the triple negative and non-triple negative breast cancer cells.Subsequently,we used immunofluorescence to detect type I collagen in cells.The experimental results have effectively validated the conclusions obtained from the polarization experiment.In this paper,four types of breast cancer tissues were obtained by NOD/SCID mouse tumorigenesis.The Mueller matrix microscopy was used to detect the backward Mueller matrix of undifferentiated breast cancer tissue and use MMPD and MMT to obtain different polarization parameters.Among them,the MMPD parameter ? and the MMT parameter t2 can effectively distinguish breast cancer tissues in different genotypes.In order to further quantify the relationship between the polarization characterization parameters and the microstructure of breast cancer tissues,the frequency distribution histogram and central moment parameters is used to analyze the Mueller matrix of the tissue.The results of the analysis of the quantitative characterization parameters of polarization were good supplement further explains the quantitative relationship between polarization parameters and microstructure.Finally,we used the immunohistochemical method to detect the type I collagen components in the tissue.The results showed that the distribution of type I collagen in the four types of breast cancer tissue was basically consistent with the results of polarization characterization parameters and reveals the differences in the dynamic remodeling of type I collagen during carcinogenesis.In summary,the Mueller matrix microscopy is applied to detect the polarization of four types of breast cancer cells and tissues.Through the quantitative analysis of the polarization experiment results,the relationship with the microstructure of breast cancer samples was obtained.A series of polarization parameters were obtained and the relationship between polarization parameters and microstructure was validated using biological experiments.This study could provide methods and theories for subsequent polarization imaging applications in cell imaging and clinical diagnosis of breast cancer tissue genotyping.Moreover,the potential ability of polarization imaging as a new method for researching the pathological process of breast cancer is revealed.