Effect Of High Expression Of Type ? Collagen A1 Chain Protein On Clinical Prognosis In Triple Negative Breast Cancer

Objective:To analyze the relationship between the expression of type ? collagen ?1chain protein(COL1A1)and clinicopathological features in triple negative breast cancer(TNBC),and to explore the effect of COL1A1 on the clinical prognosis of TNBC.Methods:A total of 148 TNBC specimens were collected from the Affiliated Cancer Hospital of Xinjiang Medical University.The relative expression of COL1A1 mRNA and protein in TNBC cells and tissues was detected by fluorescence quantitative RT-PCR and Western blot,and the expression of COL1A1 and smooth muscle actin(?-SMA)in TNBC was detected by immunohistochemistry.The relationship between the expression of COL1A1 and clinicopathological parameters and prognosis of TNBC patients was analyzed retrospectively.Results:The mRNA and protein expression of COL1A1 in MDA-MB-231 cells were 1.696 ±0.486 and 0.550 ±0.088,respectively,which were higher than those in MCF-10 A cells(1.020 ±0.231 and 0.350±0.083,P=0.032,P=0.046).The mRNA and protein expression of COL1A1 in TNBC tissues were 1.632 ±0.598 and 0.733 ±0.068,respectively,which were higher than those in paracancerous tissues(1.041 ±0.316 and 0.612 ±0.016,P=0.003,P=0.039).The high expression rates of COL1A1 and ?-SMA in TNBC tissues were 35.8% and 56.7%,respectively,which were higher than those in paracancerous tissues(16.7% and30.0%,P=0.041,P=0.037).The expression of COL1A1 was correlated with tumor size,TNM stage,lymph node metastasis,vascular invasion and ?-SMA expression of TNBC(all P<0.05).The 5-year survival rate of patients with high expression of COL1A1 was lower than that of patients with low expression of COL1A1(P<0.05).Multivariate analysis of Cox proportional hazard regression model showed that COL1A1 expression was a factor affecting the survival of TNBC patients(HR=3.735,P=0.006).Conclusion:COL1A1 is highly expressed in TNBC and is a prognostic factor in patients with TNBC.