Correlation Study On CDX-2 Expression In Colorectal Tubular Adenocarcinoma Of Three Different TCM Constitution

Objective:To investigate the correlation of CDX-2 protein expression in colorectal tubular adenocarcinoma of Qi-deficient constitution,Yang-deficient constitution and Damp-heat constitution.Methods:We randomly selected patients from February 2019 to February 2020,who underwent electronic colonoscopy and surgical treatment in the Second People's Hospital of Fujian Province,and the pathological diagnosis was tubular adenocarcinoma.Through the judgment of TCM constitution,30 cases of Qi-deficiency constitution were included in study group 1,30 cases of Yang-deficiency constitution were included in study group 2,and 30 cases of Damp-heat constitution were included in study group 3.At the same time,30 cases of healthy,Yin-yang harmonious constitution patients who without abnormality in colonoscopy were randomly selected as the normal control group.The expressions of CDX-2 in the four groups were detected by immunohistochemistry and explore the correlation between them.Results:1.There were no significant differences in gender and age between Qi-deficient constitution,Yang-deficient constitution,Damp-heat constitution and the normal control group,indicating that the differences were comparable(P>0.05).There were no significant differences in position,degree of differentiation,stage between Qi-deficient constitution,Yang-deficient constitution and Damp-heat constitution,indicating that the differences were comparable(P>0.05).2.The positive rate of CDX-2 protein in colorectal tubular adenocarcinoma(60.00%)was lower than that in normal intestinal mucosal tissues(96.67%),and the difference was statistically significant(P<0.01).3.The positive rates of CDX-2 in low differentiation,medium differentiation and high differentiation were 40.74%,66.07% and 85.74%,respectively.The difference between the three groups was statistically significant(P<0.01).the expression level of CDX-2 was positively correlated with the degree of differentiation(rs=1,P<0.01).4.The positive rates of CDX-2 in early colorectal cancer and advanced colorectal cancer were 78.26% and 53.73%,respectively.The differences between the groups were statistically significant(P<0.05).5.The positive rates of CDX-2 protein expression in the lower age group(41-60 years old)and the higher age group(61-80 years old)were 58.70% and 61.36%,respectively.The positive rates of CDX-2 protein expression in male and female were 64.72% and 53.85%,respectively.The positive rates of CDX-2 protein expression in the left colon and the right colon were 61.54% and 57.89%,respectively.The differences between the three groups were not statistically significant(P>0.05).6.Qi-deficy tubular adenocarcinoma(46.67%)?Yang-deficy tubular adenocarcinoma(50.00%)< Damp-heat tubular adenocarcinoma(83.33%)< Normal intestinal mucosal tissues(96.67%),and there were no significant differences between Qi-deficient and Yang-deficient constitution(P>0.05).The difference between Qi-deficient and Damp-heat constitution,Yang-deficient and Damp-heat constitution were statistically significant(P<0.05).Conclusions:1.The CDX-2 protein positive rate in colorectal tubular adenocarcinoma tissues was significantly lower than that in normal intestinal mucosal tissues.2.The positive rate of CDX-2 protein in advanced colorectal tubular adenocarcinoma was lower than that in early stage.3.The CDX-2 protein positive rate was positively correlated with the differentiation degree of colorectal tubular adenocarcinoma.4.The CDX-2 positive rate had no significant correlation with age,sex and tumor site.5.In colorectal tubular adenocarcinoma,the positive rate of CDX-2 protein in Qi-deficient constitution and Yang-deficient constitution were lower than that in Damp-heat constitution.