Immunomodulatory Effect On T Lymphocytes Of Mesenchymal Stem Cells Derived From Human Embryonic Stem Cells

Mesenchymal stem cells(MSC)exert their therapeutic benefit due to not only their differentiation potency but also their potent immunomodulatory properties.Embryonic stem cells(h ESC)are able to proliferate unlimitedly in vitro with the potency to differentiate into almost all types of cells in the body and thus MSC derived from h ESC(h ESC-MSC)would be a renewable,highly homogenous,and off-the-shelf MSC product.Despite of various protocols developed by other groups,a high-efficient and cost-effective h ESC-MSC differentiation protocol is still needed and,more importantly,it has been controversial for the immunomodatory function in such cells.We have recently established a simple and highly-efficient h ESC-MSC differentiation protocol.The present study investigated the immunomodulatory properties of the h ESCMSC.The results showed that h ESC-MSC had significant effects on T lymphocyte proliferation inhibition and Treg differentiation promotion;the immunomodulatory function was stronger than that of umbilical cord mesenchymal stem cells(UC-MSC).In conclusion,these h ESC-MSCs had superior immunomodulatory effects on T lymphocytes,indicating that h ESCMSC might be more suitable for the treatment in immune-related diseases.Based on the established h ESC-MSC differentiation protocol,we further prepared h ESC-MSCs that met the defined standards of mesenchymal stem cells by changing four coating materials(gelatin / collagen / hyaluronic acid / fibronectin).The immunoregulatory characteristics of h ESC-MSCs from these four sources were studied in depth.The results show that h ESC-MSCs differentiated by hyaluronic acid and gelatin matrix can inhibit the proliferation of T lymphocytes and promote the immune regulation of Treg differentiation.They are better than other coating materials and stronger than umbilical cord mesenchymal stem cells(UC-MSC).This study confirms that h ESC-MSCs differentiated by gelatin and hyaluronic acid coating materials have stronger immunoregulatory potential and are expected to become the first choice of seed cells for clinical immunotherapy;two coating materials affect the immune regulation ability of h ESC-MSC to Important links provide data support for the selection of cell culture materials in future immunoassay research,and have great application value in the fields of tissue engineering and biomedicine.