| Gout,a kind of arthritis disease caused by metabolic disorder and inflammation,is hard to cure,and the pain shows the trend of repeated attacks,which seriously affects people's quality of life.Medical researchers pay close attention to gout and have no perfect treatment plan.The continuous high level of uric acid in the body is considered as the basis of gout attack.This phenomenon is called hyperuricemia,which will cause cardiovascular and cerebrovascular diseases,diabetes and other metabolic diseases.There are two guiding directions for gout treatment,one is anti-inflammatory and analgesic,the other is to reduce uric acid and promote the dissolution and elimination of gout stone in gout affected areas.Colchicine,allopurinol and other clinical anti-inflammatory,analgesic and uric acid reducing gout treatment measures,due to its accompanying diarrhea,abdominal pain,drug allergy,high initial dosage of drugs,drug resistance and other restrictive factors,leading to the current lack of a recognized and perfect treatment measures for gout.A new,safe,less side effects or no new gout treatment program is still the direction of scientific research of relevant practitioners.Isoastilbin(IA),a small molecular dihydroflavonol glycoside isolated from natural medicine,has shown good antioxidant effect in previous studies.In this study,a rat model of gouty arthritis was established by injecting sodium urate(MSU)crystal.The anti-gout effect of IA was evaluated by evaluating the changes of swelling rate and inflammation level after IA,and the mechanism was characterized.The results showed that ia had a significant inhibitory effect on the swelling of ankle joint caused by MSU.The pathological section showed that it had a good effect on inhibiting the inflammatory cells recruitment in ankle joint caused by MSU.Moreover,ia can effectively reduce the concentration of IL-1?,IL-6,IL-8,IL-16,IFN-?,PGE2,TNF-?,MIP-1? and MCP-1 in the serum of rats with gouty arthritis;the concentration of IL-10 has been significantly increased,which effectively confirmed the activity of IA,and can be initially determined that the role of IA is related to inflammation inhibitionand regulation of IL-1? and its cascade reaction pathway.The activity and possible mechanism of IA in reducing uric acid were evaluated in the high uric acid mice system with the introduction of OXO and high purine food(yeast extract).After IA treatment,the increase of uric acid value caused by mouse modeling was significantly inhibited,and the directly related XO enzyme activity was also significantly inhibited.IA treatment also has a beneficial effect on the redox balance of hyperuricemic mice,reducing the content of oxidation-related factors in the system,and improving the activity of antioxidant enzymes such as SOD,which can effectively protect the body from oxidative damage.According to the experimental results,it can be found that the activity of IA to reduce uric acid is related to the regulation of the level of oxidation-related factors and the activity of xanthine oxidase.Based on the animal model,this study confirmed the anti-gout activity of IA,and made a preliminary study on its mechanism,which will provide a strong research basis and theoretical data basis for the development of IA as a new gout treatment drug transformation and promoting the industrialization process. |
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