| ObjectiveTo study the role of "fear" in the development of cancer,the molecular mechanism of "fear" regulating the expression of miR-21 promoting the development of GC was analyzed by taking gastric cancer as the entry point.It provides a basis for clinical attention to the psychological care and humanistic care of tumor patients.Methods:Human gastric cancer cells sgc-7901 were transplanted into BABL/c-nu male nude mice to construct a subcutaneous tumor model of human gastric cancer.The tumor mass was removed and implanted into large curvature of the stomach of BALB/c-nu male nude mice to construct an orthotopic tumor model of GC.Gastric cancer control group,sham operation group,gastric cancer fear model group and yougui pills group were set up,altogether 4 groups,6 in each group.In addition to constructing the orthotopic tumor model of GC,the nude mice in the gastric cancer fear model group and yougui pills group also constructed the model of “fear damaging kidney” by the methods of cat sound to fear the mice plum needle knocking on the head,shaking the cages,hanging on water and simulating cat's claws to take the mouse.Anti-drug-proof intervention was carried out in nude mice of yougui pills group with classic kidney-tonifying prescription.All the remaining groups were given saline of equal volume for 4 weeks.After the last administration,the samples of GC and para-carcinoma tissue were obtained and the following indexes were observed: HE staining was used to observe the morphological and structural changes of GC and para-carcinoma tissue;fluorescence quantitative PCR was used to detect the expression level of miR-21;fluorescence quantitative PCR and Western Blot were used to measure the expression levels of PDCD4,bcl-2 and Bax.Results:1.The weight of nude mice: Before modeling,there was no statistical significance in weight of each group.By the second week after the model administration,nude mice in the gastric cancer fear model group lost weight compared with those in the control group(P < 0.05).Compared with the gastric cancer panic model group,the body weight of nude mice in yougui pills group increased(P < 0.01).By the second week after the model administration,compared with sham operation group,nude mice of gastric cancer control group lost weight,but there was no statistical difference.Compared with the control group of gastric cancer,the body weight of nude mice in the gastric cancer fear model group was significantly reduced(P < 0.05).Compared with the gastric cancer fear model group,the body weight of nude mice in yougui pills group increased significantly(P < 0.05).2.Tumor weight: compared with the gastric cancer control group,the tumor weight of the gastric cancer fear model group was significantly increased(P < 0.01);compared with the gastric cancer fear model group,the tumor weight of yougui pills group was significantly reduced(P < 0.01).3.HE staining: in the gastric cancer tissues,the cancer cells in the gastric cancer control group were slightly dense,the cancer cells in the gastric cancer fear model group were densely distributed,with relatively rich blood vessels and good blood supply,and the cancer cells in the yougui pills group were relatively sparse.In the para-carcinoma tissues,the gastric mucosa boundary of the gastric cancer control group was incomplete,part of the glandular structure was disordered,and there was a little lymphocyte infiltration;in the gastric cancer fear model group,the gastric mucosa layer structure was incomplete,the glandular structure was destroyed,and lymphocytes were infiltrated;the structure of each layer of gastric mucosa in yougui pills group was slightly complete,and the glandular structure was regular compared with the control group and the fear model group of gastric cancer,with a small amount of lymphocytes infiltrating.4.Expression level of miR-21 in gastric cancer tissues: compared with the gastric cancer control group,the expression of miR-21 in the gastric cancer fear model group increased(P < 0.01);Compared with the gastric cancer fear model group,the expression of miR-21 in yougui pills group decreased(P < 0.01).5.Expression levels of PDCD4,bcl-2 and Bax genes in GC and para-carcinoma tissues:(1)Compared with gastric cancer control group,the expression levels of PDCD4,Bax mRNA and protein in gastric cancer and para-carcinoma tissues of the gastric cancer fear model group decreased(P < 0.05,P<0.01).Expression of bcl-2 mRNA and protein in gastric cancer tissues of gastric cancer fear model group increased(P < 0.05),the expression of bcl-2 mRNA in para-carcinoma tissues increased(P<0.01),and its protein expression was on the rise,but not statistically significant.(2)Compared with the gastric cancer fear model group,in yougui pills group,the mRNA and protein expressions of PDCD4 and Bax in gastric cancer tissues increased(P < 0.05),while the mRNA and protein expressions of bcl-2 decreased(P < 0.05).In the yougui pills group,the expression of PDCD4 and Bax mRNA in para-carcinoma tissues increased(P < 0.01),and the protein expression showed an increasing trend,while the expression of bcl-2 mRNA decreased(P < 0.01),and the protein expression showed a decreasing trend,but the protein expression was not statistically significant.(3)Compared to the sham group,in the gastric cancer control group,the expression of PDCD4 and Bax mRNA in para-carcinoma tissues decreased(P < 0.05),and the protein expression showed a downward trend;the mRNA expression of bcl-2 increased(P < 0.05),and the protein expression showed an increasing trend,but expression of their protein was not statistically significant.Conclusions1."Fear" may promote the proliferation and differentiation of cancer cells by up-regulating the expression of miR-21 in gastric cancer tissues of the nude mouse model of " fear damaging kidney " gastric cancer,thereby regulating the apoptosis genes PDCD4,Bcl-2 and Bax.2."Fear" play a role in promoting the progression of gastric cancer.3.Yougui pills can slow down the progression of " fear damaging kidney " gastric cancer in nude mice model. |
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