| Objective To explore the relationship between the polymorphism of E670G(rs505151)locus of the proprotein convertase subtilisin kexin type 9 gene of Enshi Tujia family and ischemic stroke with carotid atherosclerosis.To explore the genetic polymorphisms,blood lipids,and blood selenium association with the occurrence or development of stroke,and provide theoretical support for the prevention and treatment of ischemic stroke with carotid atherosclerosis in Enshi area.Methods(1)Collecting all the patients from June 2017 to August 2019 of ischemic stroke with carotid atherosclerosis were obtained from The Central Hospital of Enshi Tujia and Miao Autonomous Prefecture in the Department of Neurology,at the same time,the healthy Tujia people determined after the medical examination at the medical examination center of this hospital were the control group.including 95 cases in the case group and 86 cases in the control group.(2)All samples were subjected to polymerase chain reaction-gene sequencing.Blood lipid and blood selenium levels of samples were detected in our hospital.(3)The statistical data were analyzed by SPSS25.0 statistical software.Results(1)The levels of TC,TG,LDL-C,and ApoB in the case group were significantly higher than those in the control group,but the HDL-C is lower,and the differences were statistically significant(P<0.05).(2)Compared with the control group,the blood selenium level was lower in the case group,and the difference was statistically significant(P<0.05).(3)There was no significant correlation between blood selenium and TC,TG,HDL,LDL,ApoA,ApoB levels in case group and control group(P>0.05).(4)Multivariate Logistic regression analysis showed that:there were statistically significant between blood selenium(OR=1.024,P<0.05),TC(OR=0.011,P<0.05),LDL(OR=15.470,P<0.05)and the incidence of IS,among which LDL has the highest correlation with IS,with an OR value of 15.470.(5)The difference in LDL-C levels between AA and GG genotypes in the case group was statistically significant(P<0.05),and LDL-C levels were higher in carriers of the PCSK9 gene 670G allele.The levels of GA genotype TC,LDL-C,and ApoB in the normal control group were higher than those in the AA genotype,and the differences were statistically significant(P<0.05);The differences in TC,TG,HDL-C,LDL-C,and ApoB were statistically significant(P<0.05),but there were no significant differences in the blood lipid levels of the GA genotypes between the two groups(P>0.05).There was no significant difference in blood selenium levels between the case group and the control group and between different genotypes within the group(P>0.05).(6)Compared with the control group,the frequency of GA+GG genotype in the case group was significantly increased,and the difference was statistically significant(c~2=8.031,P value=0.018).The frequency of 670G allele was higher in the case group,and the difference was statistically significant(c~2=8.125,P value=0.004).Conclusion(1)Increased levels of TC,TG,LDL-C,and ApoB may be high-risk factors for ischemic stroke with carotid atherosclerosis in the Enshi Tu family.HDL-C maybe a protective factor for stroke.(2)Increased blood selenium levels in humans may be protective factors for cerebral infarction in the Tujia of Enshi.(3)Carriers of the PCSK9 gene 670G allele of Enshi Tujia significantly increased LDL-C levels,and TC,TG,and ApoB also increased.(4)Patients with GA+GG genotype have an increased risk of cerebral infarction.The670G allele may be a risk gene for cerebral infarction in the Enshi Tujia population.(5)The polymorphism of E670G locus of PCSK9 gene mainly exists in AA and GA types in Enshi Tujia population,and only a few people have GG type. |
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