Ischemic Stroke Disease, Blood Stasis And Candidate Gene Association Studies Of Snps

PURPOSE:To investigate the relationships between specific genetic polymorphisms and blood stasis syndrome(BSS) of ischemic stroke(IS).METHODS:The angiotensinogen(AGT) gene M235T polymorphism,apolipoprotein E(ApoE) gene polymorphism,methylenetetrahydrofolate reductase(MTHFR) gene C677T polymorphism,tumor necrosis factorβ(TNFβ) gene Thr26Asn polymorphism, angiotensinⅡtype-1-receptor(AT1R) gene A1166C polymorphism,endothelial nitric oxide synthase(eNOS) gene glu298asp polymorphism,β-fibrinogen(β-Fg) gene Arg448Lys polymorphism were studied in 89 patients with blood stasis syndrome of ischemic stroke (BSS-IS group)and 102 patients with non-blood stasis syndrome of ischemic stroke (non-BSS-IS group).Genotypes for those polymorphisms were analyzed by oligonucleotide microarray.RESULTS:The frequencies of AT1R gene C allele in patients with BSS-IS were higher than those in non-BSS-IS group(40.45%and 35.78%,respectively).AT1R gene 1166AC genotype represented a significantly increased risk for the development of BSS-IS (OR=1.866,95%CI,1.017-3.423,P=0.043).Yhe genotype distribution of eNOS gene glu298asp polymorphism were significantly different between these two groups(χ~2 =6.398, d.f.=2,P=0.041)).The frequencies of eNOS gene T allele in patients with BSS-IS were higher than those in non-BSS-IS group(23.03%and 16.18%,respectively).The eNOS gene 894TT genotype represented a significantly increased risk for the development of BSS-IS (OR=4.654,95%CI,1.255-17.259,P=0.013).CONCLUSIONS:The AT1R 1166AC and eNOS 298AspAsp genotypes maybe the risk factors associated with the susceptivity to blood stasis syndrome of ischemic stroke.