The Success Rate And In Vitro Sensibility Test Analysis Of Lung Cancer Organoids Modeling

Objective: At present,organoid techniques have shown more and more potential in preclinical models,while lung cancer organoid culture is characterized by low success rate and harsh culture conditions,so there are few reports on it.In order to explore the factors influencing the modeling of lung cancer organoid culture and to provide the basis for its clinical application,we carried out this research topic.Methods: 1.According to the inclusion criteria,a total of 126 patients with suspected lung cancer were selected and the diseased tissues were collected by means of surgery,fiberoptic bronchoscopy and CT-guided lung biopsy.2.After the diseased tissue was cleaned,the organoid model was obtained through enzymatic hydrolysis,isolation of single tumor cells,preparation of organoid culture medium,subculture and organoid database.3.According to the organoid modeling standard,the differences of the patients' general conditions,pathological sites,pathological types,pathological stages and sampling methods were statistically analyzed to obtain the relevant factors affecting the success rate of culture.4.After the successful construction of organoid model,the sensitivity of different anti-tumor drugs in vitro was detected on the enzyme marker,so as to provide evidence for clinical treatment.Results: In this study,a total of 120 lung cancer patients were enrolled,6 of whom failed to reach the standard.In the experimental group,79 of them were successfully modeled and 41 of them failed in modeling.The success rate of modeling was about 65.83%(79/120).In terms of the influencing factors of the success rate of modeling,there was no significant statistical difference in the success rate of modeling for different gender(P= 0.345),age(P= 0.680),lesion location(P= 0.731),location of upper,middle and lower lobes(P=0.246),and pathological type(P= 0.249).However,for different TNM pathological stages(P= 0.001)and different sampling methods(P=0.012),there were significant statistical differences.The sensitivity test of anti-tumor drugs in vitro showed that pemetrexed was not effective for the patient,and the single drug application of cisplatin was better than the combination of pemetrexed and cisplatin.Changchun ruibin was not effective for this patient.The combined application of vinorelbine and cisplatin showed no improvement compared with the single application of cisplatin.Gemcitabine and cisplatin were both effective for this patient,and the combined drug was better than the single drug.Both czotinib and oxitinib had inhibitory effects on the patient,and the efficacy of oxitinib was better than that of czotinib.Conclusion: The success rate of lung cancer organoid modeling was significantly higher than that of other common tumor culture models,but it was affected by TNM pathological stage and sampling method.According to the detection results of anti-tumor drug sensitivity in vitro,lung cancer organoid culture technology is expected to be an effective tool to predict drug sensitivity and guide individualized drug use.