Experimental Study Of (Natural) Horn Material Applied To Chest Wall Defects

Objective: The biocompatibility and mechanical properties of chest w all reconstruction material-natural buffalo horns after replacement of chest wall defects were evaluated.Methods: Purchase 10 healthy adult domestic dogs and buffalo horns outside the hospital.Buffalo horns were made into a size and shape similar to dog ribs.Rib defects were artificially caused during surgery.Buffalo horns were used instead of ribs.The dog's heart rate,respiratory rate,and body temperature were recorded for 3 consecutive days after surgery.Venous blood was drawn on the 1st,3rd,7th,and 14 th days after the operation,and CRP,blood routine,and liver and kidney functi-o n were measured.On the 14 th day after operation,positive and lateral radiographs of the chest were taken.At 3 months after operation,the experimental dogs were sacrificed,and the infiltration of cells,neovascula rization,and fibrous sacs at the transplantation site were observed under a microscope.Using a BOSE-3200 biomechanical testing machine and an Edberg push-pull meter,the buffalo horns after drying and in vitro were subjected to a three-point bending test and a shear test to observe changes in mechanical strength.Results:1.The clinical observation indicators of dogs within 3 days after operation are in the normal range,with a normal heart rate of 80-130 beats / min,a respiratory rate of 10-40 beats / min,and a body temperature of 38-39 °C.2.The neutrophil percentage(normal value 63-87%)of dog 2 on the first day after surgery was slightly higher than 92.8%,and the aspartate aminotransferase in dogs 1,2,and 3 were higher than normal 40 U / L 3 days before.3.There were no breaks or shedding of buffalo horns under the X-ray.4.Under the microscope,the cell infiltration in the transplantation site is mainly fibroblasts,with a small amount of monocytes and lymphocytes.Neovascularization,fibrous sacs were found in all sections,and bony buffalo horns were surrounded by epiphyses.5.The bending strength and shear strength of buffalo horns continued to decrease after transplantation,and remained basically unchanged after 7 days.Conclusions:Buffalo horn has good biocompatibility,does not cause systemic infection,hemolysis and coagulation,and has no hepatorenal toxicity.Buffalo horn can be visualized under X-ray,which is helpful for follow-up.Buffalo horns can stimulate connective tissue hyperplasia in the transplanted area and have sufficient mechanical strength so that they will not break.