Experiment Study On The Reconstruction The Chest Wall With Allograft Ribs In Rabbits

Objective Chest wall defect is a common disease, but the treatment is one of the clinical problems. More methods were used for reconstructing bone defects, and current approach is prosthetic material. This study observed the effect of allograft ribs for bone defect reconstruction, and provided a theoretical basis for clinical application.Methods (1)Made a suitable animal model of bone defect: 3.0cm×3.0cm(test 1),5.0cm×5.0cm(test 2),2.0cm×2.0cm(test 3) bone defect were created by partial rib resection without parietal pleura destruction. Breathing frequency (f) and oxygen partial pressure (PO2) were observed at preoperative, postoperative and one month later. paradoxical breathing and postoperative mortality were also observed. (2) Prepared deep-frozen allogenous ribs and created animal model of chest wall defect. Reconstructing these chest walls, allogeneic ribs (test group) and autogenous ribs (control group). The situation of the implanted chest wall prosthesis, the progress of the postoperative regeneration of the chest wall tissue, the changes of biochemical blood parameters, the changes in pathology and X-ray check were observed dynamically in those rabbits.Results (1) Both paradoxical respiration and Shortness of breath existed in test 1. No postoperative death in test 1. Breathing frequency (f) and oxygen partial pressure (PO2) had significant difference between preoperative and postoperative. No paradoxical respiration existed in one month later. postoperative death occurred within 12 hours because of Severe respiratory disordersin test 2. No paradoxical respiration occurred in test 3. (2)No perioperative death was observed in all rabbits with surgery. No postoperative chest wall subsidence and infection or paradoxical respiration occurred. Both serum alkaline phosphatases and calcium-phosphorus product had no significant difference (p=0.113 and p=0.799 respectively) in two groups. X-ray and histological check displayed a significant hyperplasy.Conclusion (1) 3.0cm×3.0cm chest wall defect can cause respiratory dysfunction such as paradoxical respiration, hypoxemia, faster respiratory frequency and so on, but no postoperative death. (2)Both allogenous and autogenous ribs can provid effective support in chest wall reconstruction, and allogenous ribs which are easily obtained can be used as prosthesis in chest wall reconstruction.