Using 16SrDNA To Observe The Structure Of Nasal Cavity In Patients With Chronic Sinusitis Of Different TCM Syndrome Types And Analysis Of The Difference Of Dominant Bacteria

Objective: 16SrDNA was used to analyze the structure of the secretion flora extracted from the complex of sinusitis and oral nasal passages in patients with chronic sinusitis of different TCM syndromes and healthy people;comparative analysis of the differences in the main dominant bacteria in the secretions of chronic sinusitis patients with different syndromes.Methods: Collected 32 patients with chronic sinusitis with different TCM syndromes(group A: 8 cases of gallbladder stagnation fever,group B: 8 cases of spleen and stomach damp heat,group C: lung meridian wind fever,group D: 8 lung and spleen qi deficiency)and The secretions of the sinus,nasal and nasal tract complexes of 8 normal people(group E)were analyzed by 16S r DNA for the diversity and structure of the nasal cavity microflora,and the comparative analysis of the predominant genus of bacteria in each group difference.Results: 1.Analyze the colonies of nasal samples from each group and find that the species diversity of group A is lower than that of other groups,and the uniformity of species is lower than that of other groups.Diversity distribution is between Group B and Groups C and D.2.The content of staphylococcus in group A was significantly higher than that in other groups and normal people(P <0.05);the content of bacilli in groups B,C and D was significantly higher than that in group A and normal people(P <0.05),and the content of staphylococcus was low In group A and normal people(P <0.05);there was no statistically significant difference in staphylococcus and other bacteria between groups B,C and D(P> 0.05).Conclusion: 1.Although the composition of the nasal flora of patients with chronic rhinosinusitis of different TCM syndromes and normal people is similar,there is a certain difference in abundance.2.There are obvious differences in the distribution of dominant bacteria in patients with chronic rhinosinusitis of different syndrome types.Chronic rhinosinusitis is a syndrome of gallbladder stagnation and heat is related to the increase of Staphylococcus epidermidis,while the incidence of other syndromes is significantly increased by the content of Bacillus,The relative reduction of Staphylococcus epidermidis content.3.Chronic sinusitis with cholestasis and heat is a clinically refractory syndrome of traditional Chinese medicine,which may have a certain relationship with the special staphylococcal flora in the local nasal cavity.