Study Of CD4~+/CD8~+ Cells Change In Peripheral Blood And Cervical Local With High-Risk Human Papillomavirus (HPV) Infection

Objective: Based on the Human Papillomavirus(HPV) infection of peripheral blood and cervical local CD4+T cells, CD8+T cells lymphocytes and the ratio of CD4+T/CD8+T change. Research the correlation between the systemic cellular immunity and the cervical local cellular immunity. Preliminary exploration of systemic cellular immunity and cervical local cellular immunity function change and the relationship with high risk HPV infects. Using antiviral treatment on cervical local or systemic provides the basis of the forecast. Looking for as cervical tissue on the immune function of HPV has the specificity of the index and criterion.Methods: Use Flow Cytometry to detect the patients’ peripheral blood CD4+ T cells,CD8+T percentage of detection and the CD4+T/CD8+T ratio of measured change; While using Immunohistochemistry(IHC) to select CD4+T cells, CD8+T cells semi-quantitative determination and CD4+T/CD8+T ratio of measured change in the cases of cervical local tissues.Results:1. HPV infection percentage of peripheral blood CD4+T cells are 37.88±8.29%, HPV-negative control group 37.46±7.01%, there is no significant different statistical analysis(P>0.05).2. HPV infection percentage of peripheral blood CD8+T cells are 30.24±6.64%, HPV-negative control group 30.91±7.18%, there is no significant different statistical analysis(P>0.05).3. HPV infection of peripheral blood CD4+T/CD8+T ratio is 1.36±0.59, HPV-negative control group 1.32±0.57, there is no significant different statistical analysis(P>0.05). HPV infection CD4+T/CD8+T>1 or <1, number of cases respectively 20 and 10, HPV-negative control group CD4+T/CD8+T>1 or <1, number of cases respectively 21 and 9. There is no statistically significant between the two group(P>0.05).4.HPV positive cervical biopsy diagnosis chronic cervicitis and cervical lesions, there is no significantly different in peripheral blood CD4+T cells, CD8+T cells and CD4+T/ CD8+T ratios between chronic cervicitis and cervical lesions(P>0.05).5.HPV-16 positive subtype with other HPV positive patients, the peripheral blood CD4+T and CD8+T and the ratio of CD4+T/CD8+T change without statistical significance(P>0.05);6. HPV infected cervical local tissue CD4+T cell number are 158.87±98.42, significantly lower than HPV-negative control group 234.07±89.42, statistical analysis of both statistically significant(P<0.05).7. HPV infected cervical local tissue CD8+ T cell number are 185.77±92.42, lower than HPV-negative control group 203.13±78.27, but statistical analysis show no significant difference between the two(P>0.05).8. HPV infected cervical local tissue CD4+T/CD8+T ratio is 0.82±0.40, significantly lower than HPV-negative control group1.25±0.58, the difference was statistically significant(P<0.05).HPV infection CD4+T/CD8+T>1 or <1, number of cases respectively 4 and 26 cases, HPV negative control CD4+T/CD8+T>1 or <1, respectively 24 and 6 cases, statistically significant(P<0.05).9. HPV positive cervical biopsy diagnosis chronic cervicitis and cervical lesions, there is statistically significant in cervical tissue CD4+T cells, CD8+T cell(P<0.05), but the ratio of CD4+T/CD8+T change without statistical significance(P>0.05).10.HPV-16 positive subtype with other HPV positive patients with cervical tissue CD4+T and CD8+T and the ratio of CD4+T/CD8+T change without statistical significance(P>0.05)11.Statistical analysis of HPV infection group and non-infected peripheral blood and cervical local tissue CD4+T、CD8+T no correlation(P>0.05).12. HPV positive in cervical biopsy diagnosis of chronic cervicitis were followed 3-6 months, HPV overcast group whose cervical tissue CD4+T cells, CD4+T / CD8+T ratio was statistically significant(P<0.05); But CD8+ T cells was not statistically significant(P>0.05).Conclusions:1. The presence of cervical HPV infection cellular immunity is low, Systemic cellular immune did not change significantly. That provide theoretical basis for the use of topical immunotherapy enhanced for cervical HPV infection.2. The body cellular immune has no correlation with cervical local cellular immunity.3. Cervical local tissue CD4+ T cell joint CD4+T/CD8+T ratio is expected to become a potential predictor of HPV infection outcome.