| Objectives To explore the effect of night light exposure,RORA and PER2 gene polymorphism and their interaction on the increase of fasting blood glucose in steel workers,to provide basis for early intervention.Methods Based on the baseline survey of the cohort study on the health status of occupation in Beijing,Tianjin and Hebei,taking 7034 on-the-job workers in Tangshan Iron and steel company who took part in the 2017 occupational health examination as the research objects,we collected relevant data and information through questionnaire survey and physical examination,the correlation between the duration of night light exposure and the increase of fasting blood glucose was analyzed by using the Restricted cubic spline model,and the influencing factors related to the increase of fasting blood glucose was also analyzed by using the multi factor Logistic regression model.The interaction of different factors was analyzed.Combined with the inclusion and exclusion criteria of the subjects,a total of 366 men with fasting blood glucose and 366 men with normal fasting blood glucose were randomly selected from 7034 persons with complete data.The DNA was extracted from the case-control group,and the target gene fragments were amplified by polymerase chain reaction.The amplified products were digested and cut by restriction endonuclease,and then agarose gel electrophoresis was used to distinguish SPSS.Logistic regression was used to analyze the effect of gene and gene,gene and environment interaction on fasting blood glucose.Results 1.The detection rate of fasting blood glucose was 62.0%(4360/7034),of which 63.3%(4080/6442)was male and 47.3%(280/592)was female;2 The correlation between the duration of night light exposure and the increase of fasting blood glucose in iron and steel workers was analyzed by the restricted cubic spline,adjust age,gender,education level,marital status,monthly family income per capita,smoking,drinking,diet mode,salt intake,sleep duration,sitting duration,physical activity,BMI,hypertension,dyslipidemia,family history of diabetes mellitus,draw curtains when sleeping,turn on the light,light intensity,light objects outside the window when sleeping,turn on the light times when sleeping,high temperature exposure,noise exposure,dust exposure,CO exposure and shift work.Showed that there was a linear dose-response relationship between the duration of night light exposure and the rise of fasting blood glucose(overall correlation test ?2=8.80,P=0.012;nonlinear test ?2=3.35,P=0.067);3 The results of multivariate logistic regression analysis showed that night light exposure and night light exposure during sleep were the risk factors of fasting blood glucose.The risk of fasting blood glucose increased by 20.1%(or = 1.201,95% CI: 1.001-1.441)when night light exposure ?4304h compared with the reference group;The results of night light exposure during sleep showed that: compared with the darkest group,the darker group increased the risk of fasting blood glucose increased by 17.6%(OR=1.176,95%CI:1.048-1.321);4 The rs8034880 polymorphism of RORA gene was associated with the increase of fasting blood glucose,and the recessive model(AA,Ag vs GG)was statistically significant(OR=1.48,95%CI:1.01-2.16);no association was found between rs2304672 polymorphism of PER2 gene and fasting blood glucose rise;5 No interaction between rs8034880 of RORA gene and rs2304672 of PER2 gene was found;6 There was an interaction between the rs8034880 polymorphism of RORA gene with the duration of night light exposure and the brightness of bedroom.GG gene can increase the risk of fasting blood glucose increase in workers with short-term night light exposure(OR=2.123,95%CI:1.087-4.144)and those with the darkest bedroom brightness(OR=2.048,95%CI:1.048-4.004);7 There was interaction between the polymorphism of rs2304672 locus of PER2 gene and the duration of night light exposure in work.GG gene could reduce the risk of fasting blood glucose increase in short-term night light exposure(OR= 0.495,95% CI:0.264-0.927),but no interaction between the locus and the brightness of bedroom was found.Conclusions 1 Exposure to night light at work and night light during sleep are the risk factors for the increase of fasting blood glucose in iron and steel workers;2 The rs8034880 gene polymorphism of RORA gene is related to the increase of fasting blood glucose in male iron and steel workers;3 The rs2304672 gene polymorphism of PER2 gene is related to the increase of fasting blood glucose in iron and steel workers;4 There is no interaction between rs8034880 and rs2304672;5 There is interaction between rs8034880 of RORA gene and night light exposure in work and brightness of bedroom;There is interaction between rs2304672 gene polymorphism of PER2 gene and night light exposure in work.Figure 8;Table 23;Reference 97... |
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