Effects Of Intraoperative Spraying Of PA-MSHA On Postoperative Immune Function In Patients With Non-small Cell Lung Cancer

Objective: To investigate the effect of PA-MSHA spraying Before the end of the thoracic surgery on the immune function of patients with non-small cell lung cancer treated by surgery,And evaluate the safety of the drug.Methods: A total of 48 patients with NSCLC admitted the third thoracic surgery department of shengjing hospital from June 2018 to June 2019 for surgical treatment were randomly divided into the experimental group and the control group.25 patients in the experimental group were sprayed with PA-MSHA 10 ml before sternal closure.In the control group,10 ml of normal saline was sprayed into the thorax before sternal closure.5ml of thoracic fluid samples were collected from the patients in each group on the first day after the operation and before the extraction of the thoracic duct,and the relevant immune indexes including il-2,il-4,INF-?,and TNF were detected.Also,5ml of venous blood was extracted from the patients in each group before and on the 5th day after surgery,and the relevant immune indexes including il-2,il-4,il-6,il-10,il-17,inf-?,and TNF were detected.Statistical methods were used to analyze the difference of relative immune indexes in pleural effusion and blood between two groups and at different times.Results: The average level of TNF in the pleural effusion of the experimental group was significantly higher than that of the control group on the first day after surgery and before pulling out the thoracic duct(P < 0.05).There was no significant difference between the experimental group and the control group in il-2,il-4,and inf-? in the pleural effusion before pulling out the thoracic duct on the first day after surgery.The mean level of IL-6in the blood of the experimental group on the fifth day after surgery was higher than that before surgery,and the difference was statistically significant(P < 0.05).So did the control group.There were no significant differences in il-2,il-4,il-10,il-17,INF-and TNF in the blood of the experimental group and the control group on the 5th day after the operation compared with the peroperative level.Compared with the control group,the immune indexes in the blood of the experimental group were not significantly improved.In the experimental group,two patients developed fever(the body temperature is more than 38.5c,without chills),which was cured after symptomatic treatment,and no obvious drug-related adverse reactions were observed.Conclusion: PA-MSHA is safe and well-tolerated.Simple chest spraying without blood injection can effectively improve the average level of TNF in postoperative pleural effusion.Furthermore,it can improve the local immunity level.This may play a certain role in reducing perioperative infection rate and inhibiting tumor cell proliferation and pleural metastasis.