Study On The Mechanism Of Anti-hyperuricemia And Renal Function Protection Of Vine Tea Extract

Objective: Hyperuricemia is a metabolic disease with uric acid abnormality as the main clinical manifestation,renal function damage is one of the most common complications of hyperuricemia,seriously endanger human health.Metabolic diseases such as diabetes and hyperuricemia are the dominant diseases for prevention and treatment of traditional Chinese medicine,especially in the early prevention and treatment of related complications,traditional Chinese medicine and minority medicine have better clinical advantages and treatment characteristics.Vine tea is a kind of medicinal food health tea commonly used in many ethnic minority areas in China,it is rich in active ingredients and nutrients.In this paper,based on the previous drug research and clinical practice feedback,the vine tea extract was taken as the research object,different hyperuricemia animal models and hyperuricemia renal function injury animal models were established respectively,to study the pharmacodynamics of the extracts of vine tea against hyperuricemia,and the protective effect on renal function of hyperuricemia and its mechanism,in order to reveal the scientific connotation of the anti-hyperuricemia and the protection of renal function of vine tea extract,guidance on clinical rational use of anti-hyperuricemia and complications,and the further health development of vine tea medicinal resources provides scientific basis.Methods:(1)Pharmacodynamic study on anti-hyperuricemia: The hyperuricemia models induced by yeast,hypoxanthine and potassium oxazinate were established respectively,in terms of drug improvement of uric acid metabolism(UA)and uric acid metabolism enzymes(XOD,ADA)efficacy indicators,systematically explore the pharmacodynamic effects of vine tea extract on anti-hyperuricemia.(2)Protective effect on renal function of hyperuricemia: Establish renal function injury models of hyperuricemia in rats and mice,from the aspects of improving uric acid metabolism(UA),uric acid metabolism enzyme(XOD,ADA),biochemical indexes of renal function(Cr,BUN),and renal histopathological changes and other multi-channel pharmacodynamic indicators,systematically explore the protective effect of vine tea extract on renal function of hyperuricemia.(3)Study on the protective mechanism of renal function in hyperuricemia: Establish a renal injury model of hyperuricemia in rats.From the perspective of anti-lipid peroxidation injury(SOD,MDA,GSH-Px and CAT)and improvement of related inflammatory pathways(TNF-?,IL-1?,IL-6 and TGF-?)and regulating the expression of urate transporters(URAT1,GLUT9 and ABCG2),the protective mechanism of vine tea extract on renal function of hyperuricemia was investigated by the combination of multiple levels,multiple approaches and multiple techniques.Results:(1)Pharmacodynamic study on anti-hyperuricemia: Vine tea extract can significantly or partially reduce the content or activity of UA,XOD and ADA in serum of different hyperuricemia mice models,showing better improvement of uric acid metabolism and anti-hyperuricemia effect.(2)Protective effect on renal function of hyperuricemia: Vine tea extract can significantly or partially reduce the content or activity of serum UA,XOD,ADA,Cr and BUN in rats and mice hyperuricemia renal function injury models.Pathological HE test also showed that vine tea extract could significantly or partially relieve the thickening of glomerular basement membrane in model rats and mice,reduce the disorder and necrosis of renal tubular arrangement,and inhibit the proliferation of fibrous tissue in the lesion area.It is suggested that vine tea extract has better pharmacological effects of improving uric acid metabolism and protecting renal function,and effectively alleviates the occurrence of complications of renal impairment caused by hyperuricemia.(3)Study on the protective mechanism of renal function in hyperuricemia: The anti-hyperuricemia and renal protection mechanism of vine tea extract may be related to the improvement of uric acid metabolism,anti-lipid peroxidation injury,inhibition of the expression of related inflammatory factors,and regulation of the reabsorption and excretion of urate.Specific performance in the following aspects:(1)Anti-lipid peroxidation injury: Vine tea extract can significantly or partially reduce the serum MDA content of rat models of hyperuricemia-induced renal impairment,increase the content or activity of SOD,GSH-Px and CAT,and shows better resistance to lipid peroxidation and elimination free radical damage to improve the antioxidant function of kidney tissue.(2)Inhibits the expression of related inflammatory factors: Vine tea extract can obviously or partially inhibit the protein expression level of TNF-?,IL-1?,IL-6 and TGF-?and in kidney tissue of model rats,showing a better inhibition of the synthesis and release of pro-inflammatory factors,reduce the formation of renal interstitial fibrosis,and effectively protect renal function and tissue.(3)Regulating the expression of urate transporter: Vine tea extract can significantly inhibit the expression of urate reabsorption related protein URAT1 and GLUT9,and promote the expression of urate excretion related protein ABCG2,it shows that the drug can reduce the reabsorption of urate,promote the excretion of urate in kidney,on the one hand,it can enhance excretion and reduce the level of blood uric acid in the body,on the other hand,it can reduce the accumulation of urate in the kidney,so as to protect renal function and renal tissue.Conclusion: In summary,vine tea extract has better anti-hyperuricemia and renal function protection.The preliminary mechanism may be related to the drug's anti-lipid peroxidation injury,improvement of related inflammatory pathway,and regulating the expression of urate transporters.