The Expression And Clinical Significance Of Interleukin-22 In Labial Salivary Glands Of Patients With Primary Sjogren's Syndrome

ObjectiveTo investigate the expression of interleukin-22(IL-22)in the labial salivary glands of patients with primary Sjogren's syndrome(pSS)and its relationship with the infiltration of lymphocytes in the gland,C-reactive protein(CRP),immunoglobulin,erythrocyte sedimentation rate(ESR),anti SSA antibody and anti-SSB antibodies.MethodsThe labial gland tissues of 30 patients with pSS and 10 controls were stained with IL-22 by immunohistochemical method.The lymphocytic infiltration in labial gland was detected by HE staining.C-reactive protein(CRP)and immunoglobulin(IgG,IgA,IgM)were detected by immunoturbidimetric nephelometry.The erythrocyte sedimentation rate(ESR)was determined by Wechsler method,and antinuclear antibody(ANA)was detected by indirect immunofluorescence.Western blot was used to detect anti-SSA and anti-SSB antibodies,The correlation between IL-22 and lymphocytic infiltration in labial gland,and the levels of ESR,CRP,immunoglobulin,anti SSA antibody and anti SSB antibody were analyzed.Data were analyzed by using t-test and Pearson correlation analysis.Results?The expression of IL-22 was high in the labial salivary glands of pSS patients,mainly in ductal epithelial cells,acinar epithelial cells and lymphocytic infiltrating areas The positive expression rate of IL-22 in pSS group was 28 cases,the positive expression rate was 93%,the positive expression of IL-22 in the control group was 1 case,the positive expression rate was 10%,and the expression rate of IL-22 in the two groups was statistically significant(t=8.73,P<0.05).? In the pSS group,lymphocytes were found to be focal or diffuse infiltration,expressed in the acinar lobules and ducts around the labial gland tissue,and the acinar structure of the labial glands was destroyed,and the acinus and ducts were atrophied to varying degrees.The number of IL-22 positive cells was positively correlated with lymphocyte infiltration(r=0.853,P<0.05).?Among the 30 patients with pSS,27 patients(90%)were positive for ANA,25 patients(83%)were positive for anti-SSA antibody,and 11 patients(37%)were positive for anti-SSB antibody.Main clinical and laboratory indicators:The courses of disease were(19 ± 15)months.ESR(60 ± 40)mm/h,CRP(11± 20)m/L,IgG(17.0 ± 9.6)g/L,IgM(1.4 ± 0.7)g/L,IgA(3.2 ± 1.5)g/L.The number of IL-22 positive cells(53±22)in autoantibody positive labial gland tissue was significantly higher than that in autoantibody negative group(11±8),the difference was statistically significant(t=-7.64,p<0.05),and there was no correlation with CRP?IgG?IgM?IgA?ESR(r=-0.112,P>0.05;r=0.233,P>0.05;r=0.011,P>0.05;r=-0.136,P>0.05;r=0.111,P>0.05).Conclusion1.IL-22 is highly expressed in the labial gland of patients with pSS,so it is speculated that IL-22 has a certain role in the pathogenesis of pSS.2.IL-22 is mainly expressed in the ductal epithelial cells,acinar epithelial cells and lymphocyte infiltration areas of the labial gland of patients with pSS,and its expression level is positively correlated with the degree of lymphocyte infiltration.IL-22 plays an important role in lymphocyte aggregation in the labial gland of pSS patients,suggesting that IL-22 is involved in the pathological process of pSS local inflammation.3.1n the pSS observation group,the expression level of IL-22 in autoantibody-positive labial gland tissue was significantly higher than that in autoantibody-negative group,suggesting that IL-22 can increase the production levels of autoantibodies SSA and SSB in serum of pSS patients.4.The expression level of IL-22 in the serology of pSS patients,and its specific mechanism of action in the pathogenesis of pSS and its relationship with other immune cells need further study.