Fluorescent Staining To Detect The Frequency Of Fungi In Normal Skin And It’s Changes In Lesions Of Inflammatory Skin Disease

BackgroundThe skin is the largest organ of the human body.It is a unique and changeable ecosystem.It has a very rich microbial community of bacteria,viruses,fungi and other microorganisms.The etiology and pathogenesis of some inflammatory dermatosis,such as psoriasis and atopic dermatitis,have not been fully elucidated,and the course of disease is mostly chronic.A number of reports have also suggested that inflammatory diseases such as psoriasis and eczema are associated with fungal infections.In addition,fluorescent staining is widely used in dermatology is more accurate than the traditional KOH wet film method.The number and morphology of fungi can be visually observed under the microscope,and it is fast,simple,and sensitive.Therefore,this study used this method to study the relationship between fungal colonization and inflammatory dermatosis.ObjectiveThe positive rate and colonization amount of fungi in different parts of normal body surface and the change of fungal carrier amount in the skin lesions of inflammatory dermatosis were identified by fluorescent staining method.MethodTo use fluorescent staining method to detect the number of fungi in the skin lesions of normal skin,eczema,psoriasis,pityriasis rosea and atopic dermatitis,collect relevant clinical information.The data was statistically analyzed using R3.5.3 software,P value 0.05 is statistically significant.Result(1)The detection rate of fungi in the scalp and oil parts of normal people was higher,95.05%and 96.26%,respectively.The detection rates of dry parts and wet parts were similar,83.16%and 83.67%,respectively,while that in plantar surface was the lowest,44.29%.The number of spores in the five parts of normal people is different and the difference is statistically significant.(2)The detection rate of fungi in sebaceous sites,dry,moist and plantar surface of eczema patients were 77.05%,68.97%,58.93%and 26.92%respectively.The detection rate of fungi and the number of spores were lower than the corresponding parts of normal people,and the difference was statistically significant.(3)The detection rate of fungi in the scalp,sebaceous area and dry area of psoriasis was 54.54%,66.67%and 47.62%,and the number of spores decreased,which was lower than that in the corresponding parts of normal people,and the difference was statistically significant.(4)The detection rate of fungi in the oil part of pityriasis rosea was 91.42%,which was lower than that in the sebaceous sites of normal people,but the difference was not statistically significant.The detection rate of fungi in dry part of pityriasis rosea group(57.89%)was lower than that in normal people(83.16%),and the difference was statistically significant.The number of spores in the oil and dry parts of normal people and pityriasis rosea patients was different and the difference was statistically significant.(5)The detection rate of fungi in the moist sites of AD patients was 77.78%,which was lower than that in the corresponding part of normal people,and the number of spores was reduced,but p value>0.05,which had no statistical significance;the detection rate of fungi in the sebaceous sites and the dry part was 66.67%and 55.00%,respectively,and the number of spores was reduced,P value<0.05,which had statistical significanceConclusionThe fungal load in the skin lesions of inflammatory dermatosis is generally reduced.If the fungal community is intervened,changing the microbial community structure of chronic inflammatory dermatosis may reduce the recurrence rate and alleviate the disease.