Correlation Analysis Between Intestinal Flora And Coronary Heart Disease

backgroundThe World Health Organization(WHO)predicts that cardiovascular disease will continue to be the leading cause of death in 2030,affecting approximately 23.6 million people worldwide,especially coronary heart disease,which is an important public health problem in many developing countries.In the United States,one in three deaths is due to cardiovascular disease,and in Europe,one in four deaths is due to cardiovascular disease.The results of epidemiological studies have identified a number of major risk factors leading to the development of coronary heart disease,including hypertension,hyperlipidemia,insulin resistance,and obesity.The intestinal microbial ecosystem can be said to be the largest endocrine organ of the human body,capable of producing a wide range of biologically active compounds,which can be transmitted through circulation and distributed to remote locations within the host,thereby affecting different basic biological processes of the host.Over the past decade,there is increasing evidence that the function and changes in the gut microbiota are related to cardiovascular disease.This article discusses the relationship between the gut microbiota and the disease environment and the potential role of the gut microbiota as a new therapeutic target for coronary heart disease.ObjectiveHigh-throughput sequencing was used to detect the level of intestinal flora,and real-time PCR was used to detect probiotics and harmful bacteria.Combining serum lipids and homocysteine levels,the correlation between intestinal flora and coronary heart disease was analyzed.Methods50 patients with coronary heart disease diagnosed by coronary angiography and 30 healthy subjects were selected as the control group.Approximately 5.0 g of the subject's fresh stool was placed in a sterile frozen test tube,and it was sealed and sent to the laboratory of the heart center within 2 hours.2.0 g of stool was weighed and stored in a-80 ? ultra-low temperature refrigerator.When collecting stool samples,urine or environmental pollution is avoided.High-throughput sequencing was used to detect intestinal flora,and real-time PCR was used to detect lactic acid bacteria,bifidobacteria,and E.coli.Take 5 m L of cubital venous blood on the same day or the next day,and test the serum total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),triglyceride(TG),and high density lipoprotein cholesterol(HDL-C),Homocysteine(HCY)levels.Analyze the correlation among subjects' cholesterol,triglycerides,homocysteine,and intestinal flora.Results1.Compared with the subjects in the control group,the serum levels of TC,LDL,HCY,and TG in the coronary heart disease group were significantly increased,and the levels of HDL were significantly reduced,the difference was statistically significant(P<0.05).2.Compared with the control group,the abundance of Bacteroides in the coronary heart disease group was significantly lower than that in the control group(P<0.05).There was no significant difference in the relative abundance of Actinomycota and Fusobacterium in the two groups(P>0.05).3.Compared with the normal control group,the bifidobacterium and lactobacillus in the coronary heart disease group were significantly less than the control group(P<0.05),colibacillus increased significantly(P<0.05).4.Compared with the subjects in the control group,the relative abundance of Bacteroides of the subjects with coronary heart disease and blood TC(r=-0.514,P<0.05),TG(r =-0.393,P<0.05),HCY(r=-0.498,P<0.05)showed a negative correlation.The relative abundance of Pachybacillus was related to blood TC(r=0.474,P<0.05),TG(r=0.311,P<0.05),LDL(r=0.283,P<0.05),HCY(r=0.407,P<0.05)showed a positive correlation,and the relative abundance of proteus was significantly positively correlated with TG(r= 0.309,P<0.05)and HCY(r=0.378,P<0.05)relationship.5.Compared with the normal control group,coronary heart disease group had a significant negative correlation with TG and bifidobacteria(r=-0.703,P<0.05)and a moderately positive correlation with E.coli(r=0.471,P<0.05).TC was significantly negatively correlated with bifidobacterium(r=-0.726,P<0.05);HCY was significantly negatively correlated with bifidobacterium(r=-0.712,P<0.05),and was positively and moderately correlated with colibacillus(r=0.456,P<0.05);LDL was weakly positively correlated with lactobacillus(r=0.280,P<0.05);HDL was moderately positively correlated with lactobacillus(r=0.626,P<0.05),and bifidobacterium(r=0.334,P<0.05)showed a weak positive correlation,and moderately negative correlation with colibacillus(r=-0.433,P<0.05).Conclusion: The intestinal flora may participate in the progression of cardiovascular disease,regulate blood lipids and homocysteine and other risk factors,and provide a new research direction for the further prevention and treatment of cardiovascular disease.ConclusionIntestinal flora may be involved in the development of coronary heart disease,regulate the risk factors of coronary heart disease,such as blood lipid and homocysteine,and provide a new research direction for further prevention and treatment of coronary heart disease.