Expression Of MiR-182 And Ki-67 In TypeⅠ And Type Ⅱ Endometrial Carcinoma

Background:Endometrial cancer(EC)is the second most common gynecological cancer in the world.According to Siegel et al.,in the United States,there were 63230 new cases of EC in 2018,and 11350 people died of the disease.With the improvement of living standard and the change of advanced lifestyle,the incidence rate of endometrial cancer in China is increasing year by year.The expression of Ki-67,an extensively investigated marker of cell proliferation,relects the proportion of malignant cells and is associated with tumor progression and metastasis,as well as prognosis.It has been confirmed that microrna182(miR-182)is significantly increased in EC tissues,suggesting that miR-182 plays a role in the occurrence and development of EC,but it has not been reported whether there is a difference in the expression of miR-182 in type I and type Ⅱ EC.Therefore,people began to explore the mechanism of microRNA and different types of EC,so as to improve the survival rate of EC.Objective:1.To analyze the expression of miR-182 in healthy control group and endometrial carcinoma type I and II.2.To investigate the expression of Ki-67,miR-182 in typeⅠandⅡEC and its clinical significance.Methods:The expression of estrogen receptor(ER),progesterone receptor(PR)and Ki-67 in normal endometrial tissue and EC tissue were detected by immunohistochemistry and real-time fluorescence quantitative PCR.The relationship between the expression of Ki-67and miR-182 and the clinicopathological characteristics of EC was analyzed.Results:1.1The positive expression rate of ER in typeⅠandⅡEC was 94.33%(50/53)and13.33%(2/15)respectively,and the positive expression rate of PR was 96.23%(51/53)and13.33%(2/15)respectively.The positive expression rate of ER in typeⅡEC was significantly lower than that in typeⅠEC(χ~2=42.637,P=0.00);the positive expression rate of PR in typeⅡEC was significantly lower than that in typeⅠEC(χ~2=46.725,P=0.00).1.2 The positive rate of Ki-67 was 83.02%(44/53),93.33%(14/15)and 20.00%(14/70)in type I and type Ⅱ EC tissues and normal endometrium tissues,respectively.The positive rate of Ki-67 in type I and type Ⅱ EC tissues was significantly higher than that in normal endometrium tissues(χ2=58.933,P=0.00).The positive rate of Ki-67 in type Ⅱ EC tissues was significantly higher than that in type I EC tissues(χ2=14.765,P=0.020).The expression of Ki-67 in EC tissues was related to the histological grade,histopathological stage,ER and PR expression of EC cells(P<0.05),but not to menstruation(P>0.05).1.3 One point three the relative expression of miR-182 was 3.01±0.39 and 3.32±0.20 in type I and type Ⅱ EC,and the relative expression of miR-182 in type I and type Ⅱ EC was significantly higher than that in normal endometrium.The relative expression of miR-182 in type Ⅱ EC was significantly higher than that in type I EC(Z=-2.969).The expression of miR-182 was related to the clinical stage of EC and the positive expression of ER and PR(P<0.05),but not to the menstruation of patients,the histological grade of EC and the expression of Ki-67(P>0.05).Conclusion:The expression of miR-182 and Ki-67 in EC was up-regulated,and the expression of miR-182 and Ki-67 in ECⅡwas higher than that in ECⅠ.The high expression of miR-182 and Ki-67 suggested that the prognosis of the patients was poor.