Expression Of Axin Gene In Ane-induced Rat Oral Mucosal Epithelial Cells And Its Regulatory Role Of Traditional Chinese Medicine

Objective:In this study,q-pcr,Western blot and other methods were used to investigate the effect of jiaodan xuankoukang on the oral mucosal epithelial cells of rats stimulated by areca extract(ANE)and the effect on the expression levels of Axin and n-catenin.Method:1.Fifteen SD rats were randomly divided into three groups: group A:Distilled water,group B: low dose group with jiaodan xuankoukang,and group C:high dose group with jiaodan xuankoukang,with 5 rats in each group.Group A,B and C were administered with normal saline,low dose and high dose respectively for 1week.The rats were sacrificed and serum was collected.2.The rat oral mucosal epithelial cells were purchased from sai pak and cultured under the microscope.When the cell paving rate reached 80%,trypsin was used to decompose the cells into cell suspension and divide the cells into 4 groups: Normal oral mucosa epithelial cells in rats of group A + 2% epithelial cell culture completely,group B 50 ug/ml ANE of the oral mucosa epithelial cells of rats induced by drug serum + 2% + Distilled water epithelial cell culture completely,50 ug/group C of the oral mucosa epithelial cells of rats induced by ml ANE + low-dose jan.1994-mar.1996: as dept.manager xuan mouth kang drug serum + 2% epithelial cell culture completely,group D 50 ug/oral mucosa epithelial cells of rats induced by ml ANE + high-dose jan.1994-mar.1996: as dept.manager xuan mouth kang drug serum + 2% epithelial cell culture completely,The morphology of epithelial cells in each group was observed after ANE induction for 24 h,and the m RNA and protein expressions of Axin,hydro-catenin in epithelial cells were detected by q-pcr,western blot and other methods.Results:(1)after detection,the intercellular bridge of the oral mucosal epithelial cells of rats stimulated by ANE was sparse and disappeared,abnormal mitosis increased and cell density decreased compared with that of normal rat epithelial cells,with statistically significant differences(P<0.05).The epithelial cells of the rats treated with jiaodan xuankoukang showed close cell connections,decreased abnormal mitosis and increased cell density(P<0.05),and the epithelial cell density of the high-dose group with jiaodan xuankoukang was higher than that of the low-dose group.(2)q-pcr and WB results showed that the expression of Axin m RNA and protein in the high-dose and low-dose danxuankoukang group was significantly higher than that in the ANE group,and the expression of hen-catenin protein in the high-dose danxuankoukang group was significantly lower than that in the ANE group,with significant differences(P<0.05)and the lowest expression of hen-catenin protein in the high-dose danxuankoukang group.Conclusion:1.50ug/ml ANE can promote the apoptosis of rat oral mucosal epithelial cells,and induce abnormal mitosis in normal cells by sparse and absent intercellular Bridges.Jiaodan xuankoukang can inhibit epithelial cell apoptosis,reduce mitosis,and improve the symptoms of OSF cancer.2.Axin in rats induced by 50 ug/ml ANE of the oral mucosa epithelial cells in the lower expression of beta-catenin in ANE of the epithelial cells of rats induced by high expression,prompt Axin,beta-catenin associated with OSF canceration occurred,plus jan.1994-mar.1996: as dept.manager xuan mouth,can strengthen Axin expression in the epithelial cells of rats induced by ANE,lower the amount of beta-catenin expression,and related to the dose,the higher the dose,express the stronger Axin,beta catenin expression of the less quantity.