The Effect Of Tangcaopian On Bone Marrow Suppression Of Mice Induced By Zidovudine

Background and Objective:Zidovudine is the first choice of essential drugs in the treatment of AIDS.Its antiviral effect is obvious,but the adverse reactions of bone marrow suppression often lead to the reduction or termination of the use of medication in patients,affecting the therapeutic effect.Tangcaopian,the only Chinese patent medicine approved for the treatment of AIDS,can delay the replication of the virus,increase the number of CD4+T lymphocytes in patients and improve symptoms.It has been found that Tangcaopian can promote the recovery of bone marrow suppression caused by chemotherapy drug,but whether it has the same effect on bone marrow suppression induced by zidovudine is not clear.Tangcaopian and zidovudine are anti-AIDS drugs with different mechanisms of action,if combined use could reduce the adverse reactions of zidovudine,then it might improve the clinical efficacy of anti-AIDS and increase the safety of medication.Therefore,in this experiment,a mice model of bone marrow suppression was established to study the effect of Tangcaopian on bone marrow suppression induced by zidovudine.Methods:(1)Different doses of zidovudine(500/1000/1500/2000 mg/kg)were given to mice by gavage.The appropriate dose of zidovudine for the establishment of bone marrow suppression model and the stability of the model were studied by analyzing the mortality,peripheral hemogram,number of bone marrow nucleated cells,organ index and other aspects in mice.(2)Different doses of Tangcaopian(500/1000/2000 mg/kg)and zidovudine(1000 mg/kg)were given to mice by gavage.The effect of Tangcaopian on bone marrow suppression of mice induced by zidovudine(1000 mg/kg)was studied by analyzing the general status,peripheral hemogram,number of bone marrow nucleated cells,organ index,histopathological examination of bone marrow,detection of hematopoietic growth factor(G-CSF,EPO)content and other aspects in mice.(3)Try to simulate the bone marrow suppression induced by long-term administration of zidovudine in clinical practice.Different doses of Tangcaopian(500/1000/2000 mg/kg)and zidovudine(500 mg/kg)were given to mice by gavage.The effect of Tangcaopian on bone marrow suppression of mice induced by zidovudine(500 mg/kg)was studied by analyzing the general status,peripheral hemogram,number of bone marrow nucleated cells,organ index,histopathological examination of bone marrow and other aspects in mice.Results:(1)Zidovudine administered at doses of 1000,1500,2000 mg/kg for 28 days or 35 days can significantly inhibit the hematopoietic function of bone marrow in mice.By analyzing mice mortality and the number of whole blood cells,zidovudine administered at a dose of 1000 mg/kg for 28 days is suitable for the establishment of mice bone marrow suppression model with good stablity.(2)Zidovudine,which administered at a dose of 1000 mg/kg for 28 days can significantly inhibit the hematopoietic function of bone marrow in mice,combined with different doses of Tangcaopian can improve,at varying degrees,the mice's general status,peripheral hemogram and injury of bone marrow tissue,and increase the number of bone marrow nucleated cells,organ index and the content of serum hematopoietic growth factor(G-CSF).(3)Zidovudine,which administered at a dose of 500 mg/kg for 70 days can significantly inhibit the hematopoietic function of bone marrow in mice,combined with different doses of Tangcaopian can improve,at varying degrees,the mice's general status,peripheral hemogram and injury of bone marrow tissue,and increase the number of bone marrow nucleated cells,organ index.Conclusions:(1)Zidovudine can be used to establish a stable mice model of bone marrow suppression.(2)Tangcaopian can promote the recovery of bone marrow suppression induced by zidovudine in mice.