Survivin Expression In Tongue Squamous Cell Carcinoma And Its Relationship With Epithelial Mesenchymal Transformation

Objective: To observe the expression of Survivin and the epithelial-mesenchymal transition(EMT)associated protein E-cadherin and N-cadherin in tongue squamous cell carcinoma(TSCC),and to further analyze the relationship between the above factors and the clinicopathological characteristics of TSCC.In this way,the correlation of the three expressions in TSCC was discussed to provide clinical data for the research on the occurrence and development of TSCC.Methods: The expression of Survivin and EMT markers E-cadherin and N-cadherin in63 cases of TSCC tissues and normal adjacent tissues were detected by immunohistochemical methods.SPSS20.0 statistical software was used to analyze the relationship between the expression of the three factors and clinicopathological parameters in TSCC,as well as the expression of the three factors in TSCC.Results:1.In TSCC tissues,Survivin was positively located in the nucleus,and the expression rate of Survivin in TSCC tissues(81.0%)was significantly higher than that in paracancer normal epithelial tissues(15.9%)(P < 0.05).E-cadherin is mainly expressed in the cell membrane,in the tissue of TSCC expression rate(42.9%)was significantly lower than the normal epithelial tissue of the expression rate(93.7%)(P < 0.05),Ncadherin expression in the cytoplasm and cell membrane and(or)the positive expression rate(69.8%)is significantly higher than the normal epithelial tissue adjacent to carcinoma in the expression rate(28.6%)(P < 0.05).2.According to clinical stage,the positive expression rate of Survivin in stage I-IITSCC tissues(70.3%)was significantly lower than that in stage III-IV tissues(96.2%)(P < 0.05).According to the pathological classification,the positive expression rate of Survivin in grade I TSCC tissues(54.5%)was significantly lower than that in grade II(90.5%)and grade III(100.0%)(P < 0.05).According to the presence or absence of lymph node metastasis,the positive expression rate of Survivin in TSCC tissues without lymph node metastasis(69.2%)was significantly lower than that in tissues with lymph node metastasis(100.0%)(P < 0.05).The positive expression rate of E-cadherin in stage I-II TSCC tissues(54.1%)was significantly higher than that in stage III-IV tissues(26.9%)(P < 0.05).The positive expression rate of E-cadherin in grade I TSCC tissues(81.8%)was significantly higher than that in grade II(28.6%)and grade III(15.0%)(P< 0.05).The positive expression rate of E-cadherin in TSCC tissues with lymph node metastasis(20.8%)was significantly lower than that in tissues without lymph node metastasis(56.4%)(P <0.05).The positive expression rate of N-cadherin in stage I-II TSCC tissues(56.8%)was significantly lower than that in stage III-IV tissues(88.5%)(P < 0.05).The positive expression rate of N-cadherin in grade I TSCC tissues(40.9%)was significantly lower than that in grade II(76.2%)and grade III(95.0%)(P < 0.05).The positive expression rate of N-cadherin in TSCC tissues without lymph node metastasis(56.4%)was significantly lower than that in tissues with lymph node metastasis(91.7%)(P < 0.05).In addition,there was no statistical difference in the expression of age,gender and tumor diameter(P>0.05).3.In TSCC tissues,Survivin expression was negatively correlated with E-cadherin expression(r=-0.315,P=0.012),while positively correlated with N-cadherin expression(r=-0.474,P < 0.001).Conclusion:1.Survivin is highly expressed in TSCC tissues,and low or not expressed in normal adjacent tissues,and its expression level is positively correlated with clinical stage,pathological grade,and lymph node metastasis,suggesting that overexpression of Survivin may be a risk factor for the development of TSCC.2.E-cadherin expression is down-regulated in TSCC tissues,and N-cadherin expression is up-regulated,and the expression levels of both are related to clinical stage,pathological grade,and lymph node metastasis,suggesting that EMT may occur in the development of TSCC.3.Survivin is correlated with E-cadherin and n-cadherin in TSCC,suggesting that Survivin may participate in and influence the EMT process,and the expression of E-cadherin and N-cadherin may also be regulated by Survivin.Survivin may become a new target for the treatment of malignant tumors in the future.