Single Nucleotide Polymorphisms In Chronic Cerebral Hypoperfusion And Acute Ischemic Stroke

Objective: To reveal the possible differences in the course of stroke and non-onset by means of single nucleotide polymorphisms(SNPs)approach in patients with chronic cerebral hypoperfusion and acute ischemic stroke and provide potential experimental evidence for risk prediction and early intervention to acute ischemic stroke.Methods: 37 patients(15 males)with chronic cerebral hypoperfusion and 46 patients(30 males)with acute ischemic stroke were included during their visit to our hospital from March 2018 to March 2019.Venous blood 3-4ml collected from all patients using EDTA anticoagulation tube was used to extract DNA.Genome-wide association study(GWAS)between two groups was performed with Infinium HTS gene chip from illumine.All data including age,gender,smoking,drinking,blood pressure,blood lipids,blood glucose,clinical manifestation,imaging examination and GWAS were analyzedResults: 1)In addition to gender(p=0.025),there were no statistical differences in age,smoking,drinking,blood pressure,blood lipids,and blood glucose(p> 0.05);2)Most of the clinical manifestations in chronic cerebral hypoperfusion were dizziness(18/37),headaches,memory deterioration and insomnia.Symptoms lasted three to six months(19/37)and a few patients seek medical treatment after symptoms lasting more than two years.All patients with acute ischemic stroke appeared acute symptoms such as lateral weakness of limbs,language disability,sensory disorders.3)In patients with chronic cerebral hypoperfusion,most lesions(CT/MRI examination)appeared in the side of lateral ventricle,centrum semiovale,basal ganglia,and frontal lobe(ischemic lesions,no infarction).Lesions were mostly located in the side of lateral ventricle,basal ganglia,cerebral cortex,brain stem,and even multi-site infarction in acute ischemic stroke;4)There was no significant difference between two groups in SNPs analysis.Further compared with male and female in chronic cerebral hypoperfusion,26 different SNPs were found(p<0.05)which distributed on chromosomes 1,2,3,12,15,19,23.And 5 of them were significantly different(p<0.001)which located on chromosome 23.35 different SNPs were found(p <0.05)in acute ischemic stroke between male and female.The distribution of chromosomes was same as chronic cerebral hypoperfusion including 1,2,3,12,15,19,23.However,7 of them located on chromosome 23 were significantly different(p<0.001).5)RFTN1,ARSF,PPP1R12 B,ALG10B and PCDH11 X were differential genes.PCDH11 X was a unique differential gene for acute ischemic stroke and RFTN1,ARSF,PPP1R12 B,and ALG10 B were the common differential genes for chronic cerebral hypoperfusion and acute ischemic stroke.Conclusion:1)The incidence of cerebrovascular diseases has gender difference.2)Single nucleotide polymorphisms may be genetic factors of chronic cerebral hypoperfusion and acute ischemic stroke.3)RFTN1,ARSF,PPP1R12 B,ALG10B and PCDH11 X are differential genes which have gender difference.And they could be used as candidate genes for other study on the relationship between chronic cerebral hypoperfusion and acute ischemic stroke,which can predict the incidence of cerebrovascular diseases in male and female and provide prediction methods and prevention targets.