Study On Urine Metabolism Of Non-bacterial Prostatitis With Wet-heat Betting Syndrome

Objective:The collected urine was analyzed by gas chromatography-mass spectrometry(GC-MS),and endogenous microbial chemical markers were screened.By analyzing various related urine metabolic pathways and looking for specific or differential metabolites,the main pathogenesis of damp-heat downward flow in chronic nonbacterial prostatitis(CNP)is discussed.Methods: The urine samples of 20 CNP patients who met the common diagnostic criteria of traditional Chinese medicine and western medicine collected from the outpatient department of our hospital were taken as the experimental observation group,while the urine samples of 20 healthy men in the same period were taken as the experimental normal group,aged between 20 and 50 years old.the obtained samples were analyzed by GC-MS for metabonomics,and assisted by principal component analysis,orthogonal partial least squares discriminant analysis,partial least squares discriminant and other statistical methods,according to multiple changes,t test for validation analysis.Results:Compared with the healthy group,the contents of L-proline,glucose-6-phosphate,inosine,?-aminobutyric acid,sarcosine,phosphate,glycine,ornithine,uric acid,L-glutamic acid,pyruvic acid,lactic acid,L-phenylalanine and other substances in the observation group were significantly increased.However,the contents of glutamine,malic acid and urea in the observation group decreased significantly.Conclusion: There are different metabolic differences between urine and normal healthy male urine samples in CNP damp-heat infusion syndrome,which indicates that the body of the disease has energy,sugar metabolism and amino acid disorders.