An Experimental Research On The Effect Of Capsules For Lumbago And Scelalgia Upon The Hyperpathia Effect Of Rat Model Dorsal Root Ganglion In Lumbar Disc Herniation

Objectives: The paper aims to analyze the influence the capsules for lumbago and scelalgia(CLS)have on the dorsal root ganglion(DRG)hyperpathia effect,through observing the mechanisms driving hyperpathia of rat model DRG in lumbar disc herniation(LDH).In the meanwhile,preliminary explorations on the principles of the CLS posing upon DGR hyperpathia effect are made based on p38MAPK/NF-kB signaling pathway.Methods: 80 SPF SD rats are selected,half male and half female,and they are divided into 8 groups randomly,namely,CLS low,medium,high dosages group,blank group,sham operation group,model group,SD203580 inhibitor group,PDTC inhibitor group,and there are 10 rats in each group.Except for the blank group and sham operation group,other groups equally make autotransplantation of nucleus pulposus LDH rat models.Before and after self-control comparison method is applied to detect the paw withdrawal threshold induced by hot pain,in order to identify the occurrence of hyperpathia effect and the analgesia effect of CLS.Besides,DGR of segment L4-6 is selected to measure the p38 MAPK,p-p38 MAPK,p65,p-IKK? protein level in DRG by using double immunoflurescent staining method.Western blot technique is adopted to test p38 MAPK,phosphorylation p38 MAPK,p65,IKK?,the expression of phosphorylation IKK? protein,p38 MAPK,IKK?,IL-6,the expression of TNF-? gene in DRG in each group are tested through RT-PCR technique.Results:(1)One day after modeling,the paw withdrawal threshold induced by hot pain test of rats in model group,CLS low,medium,high dosages group,SD203580 inhibitor group,PDTC inhibitor group have all decreased compared with the threshold a day before modeling.(2)The p38MAPK?p-p38MAPK?p65?IKK?and p-IKK?protein and P38 MAPK,P65 gene expression in model group rats DRG organization are apparently higher than those in blank group,but lower than those in CLS low,medium,high dosages group,SD203580 inhibitor group,and PDTC inhibitor group.(3)IL-1??IL-6?TNF-?gene expressions in model group rats DRG organization are higher than those in blank group,but lower than those in CLS low,medium,high dosages group,SD203580 inhibitor group,and PDTC inhibitor group.Conclusions:(1)Through rat autotransplantation of nucleus pulposus,the mechanism driving the DRG hyperpathia effect of LDH patients is simulated,and its molecular mechanism is likely to be relevant to the emergence of inflammatory microenvironment and the activation of p38MAPK/NF-kB signaling pathway.(2)The mechanism driving the treatment of DRG hyperpathia of LDH patients by CLS is likely to be relevant to the influence of inflammatory microenvironment.(3)The regulation mechanism driving the treatment of DRG hyperpathia effect of LDH patients by CLS is probably relevant to its regulation and control over p38 MAPK and NF-kB signaling pathways.