| Objective: To quantitatively measure the clinical periodontal index of patients with CP,RAU,and CP with RAU,and to test the levels of IL-17(Th17 factor)and TGF-?1(Treg factor)in gingival crevicular fluid and peripheral blood.To explore the pathogenesis of CP and RAU and the possible immunological association between periodontal lesions and RAU,and provide a new theoretical basis for the clinical immunotherapy of the two diseases.Methods: A total of three indicators were analyzed in this study,including periodontal index(including PD,CAL and BOP positive rate),gingival crevicular fluid and serum IL-17 content and TGF-?1 content.The experimental group was from January 2018 to In December 2018,he was first admitted to the Department of Stomatology of Shengjing Hospital affiliated to China Medical University and met the criteria of CP,RAU and CP combined with RAU(CR group).The healthy control group was from January 2018 to December 2018 in China.20 patients with non-CP and / or RAU patients and or volunteers who were first admitted to the Department of Stomatology,Shengjing Hospital affiliated to Medical University.The subjects who met the exclusion criteria were randomly selected and entered into groups.There were no significant statistical differences in age and gender composition between the 20 groups in each group(P>0.05).Firstly,the periodontal index of each group of patients was analyzed clinically.The SPSS20 software was used to analyze the periodontal pocket probing depth(PD),connective tissue attachment level(CAL)and probing hemorrhage(BOP)positive rate.Periodontal disease status of each group of patients.Secondly,11 subjects were randomly selected from each group,and the age and gender differences were excluded.Double-antibody sandwich enzyme-linked immunosorbent assay(ELISA)was used to measure gingival crevicular fluid in all subjects in group H,CP,RAU and CR.The IL-17 content in serum was statistically analyzed using SPSS20 software.Finally,the levels of TGF-?1 in gingival crevicular fluid and serum of all subjects in H group,CP group,RAU group and CR group were determined by double antibodysandwich enzyme-linked immunosorbent assay(ELISA).The data were collected by SPSS20 software.deal with.Results: PD,AL and the positive rates of BOP in the CR group were significantly higher than those in the H group,the RAU group and the CP group(P<0.05).The serum IL-17 levels in the CP group,the RAU group and the CR group were significantly higher than those in the H group(P<0.05),while CR Serum IL-17 levels in the group were significantly higher than those in the CP group and the RAU group(P<0.05).Except the CR group,the IL-17 levels in the other three groups were too low,which were not accurately detected.The levels of serum TGF-? 1 in CP group,RAU group and CR group were significantly higher than those in group H(P<0.05).There was no significant difference in serum TGF-?1 between CP group,RAU group and CR group(P>0.05).Because the levels of TGF-?1 in the four groups of gingival crevicular fluid were too low,their contents were not accurately determined.Conclusion:1.The degree of periodontal disease in patients with CP with RAU is significantly higher than that in patients with periodontitis alone,indicating that RAU may aggravate the destruction of periodontal tissues by active periodontitis.2.The effect of RAU on the destruction of periodontal tissue in chronic periodontitis may be achieved by stimulating the pathway of IL-17 synthesis in patients.3.CP patients may also be more susceptible to RAU because of long-term IL-17 overexpression,and more difficult to heal.4.As an anti-inflammatory factor,the expression of TGF-?1 may differ to a large extent at different stages of CP and RAU. |
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