Relationship Between Expression Of Stat5 And HPV16 Load And Its Changes In Cervical Lesions

Objective: Cervical cancer is the second largest cancer in women worldwide,and high-risk HPV infection is the biggest risk factor for cervical cancer.At the same time,cervical cancer is also a multifactorial disease,which is the result of interaction between virus and host.Signal transduction pathway protein-5(STAT5)is closely related to the development of cancer.In this study,we mainly explored HPV16.The relationship between STAT5 protein and cervical lesions.Methods: In this study,clinical cervical pathology was selected as the research object.A total of 140 cervical specimens were selected,including 25 normal cervical tissues,35 low-grade squamous intraepithelial lesions(LSIL),and 55 highly squamous intraepithelial lesions(HSIL),25 cases of cervical cancer tissue.The expression levels of Stat5 a,Stat5b and phosphorylated Stat5(p Stat5 a,p Stat5b)were detected by immunohistochemistry in different degrees of cervical lesions.One way-ANOVA was used for differential analysis.In addition,DNA was extracted from HPV16-positive cervical lesions and used as a template for quantitative PCR to identify HPV16 viral load in cervical lesions.The relationship between different grades of cervical lesions and HPV16 viral load was analyzed and correlated with the expression of Stat5.Results: The expression of Stat5 a in low-grade squamous intraepithelial lesions was not significantly different from that in normal tissues,but its expression level increased with the degree of progression of cervical lesions,showing a positive correlation(P<0.01).The proportion of Stat5 a high expression in normal cervical tissues,LSIL,HSIL,and cervical cancer groups was 25.0%(5/20),13.3%(4/30),68.7%(33/48),and 96.0%(24/25),respectively;Stat5b expression was not significantly different between normal cervical tissue and low-grade squamous intraepithelial lesions,but the expression level also increased with the degree of progression of cervical lesions,a positive correlation(P <0.01).The proportion of Stat5 b high expression in cervical normal tissues,LSIL,HSIL,and cervical cancer groups was 17.4%(4/23),6.2%(2/32),47.1%(24/51),and 86.9%,respectively./23);p Stat5 a expression decreased with the degree of progression of cervical lesions,a negative correlation(P <0.01).The proportion of high expression of p Stat5 a in cervical normal tissues,LSIL,HSIL,and cervical cancer groups was 96.0%(24/25),84.8%(28/33),44.9%(22/49),and 16.0%,respectively./25);p Stat5 b expression decreased with the degree of progression of cervical lesions,a negative correlation(P <0.01).The proportion of high expression of p Stat5 b in normal tissues of cervix,LSIL,HSIL,and cervical cancer was 80.9%(17/21),62.8%(22/35),34.8%(16/46),and 0.0%(0/23);Analysis of HPV16 viral load in cervical lesions revealed that HPV16 content gradually increased with the progression of cervical lesions.In quantitative PCR,we used GAPDH as an internal reference and max ?CT-?CT to evaluate HPV16 in cells.The content of max ?CT is 20,and the mean values of max ?CT-?CT in LSIL,HSIL,and cervical cancer groups are 11.94±0.68,14.44±0.48 and 18.56±1.24,respectively.Statistical analysis varies with the cervix.The progression of the lesion is gradually increasing.In addition,correlation analysis of HPV16 viral load with Stat5 protein was performed in the same specimen and found to be significantly correlated at the phosphorylation level.Conclusion: In this study,it was found that with the progression of cervical lesions,the expression of Stat5 a,Stat5b gradually increased,while its phosphorylation level decreased.Moreover,the expression of Stat5 in cervical tissues is related to HPV viral load,indicating that the progression of cervical lesions may be related to Stat5 protein in HPV16 infection,which may provide a new target for the prevention or treatment of cervical lesions.