Effect Of DIP2B On Tumorigenesis Of B16 Cells And Its Role In Adipose Tissue Development

DIP2B is a member of the DIP2 protein family encoded by the Dip2 b genes.Based on genome-wide sequencing studies,a significant association was found between Dip2 b and tumors.However,the specific role of Dip2 b in the process of tumorigenesis is not clear.The clarify the relationship and mechanism between Dip2 B and tumorigenesis can provide basis for clinical treatment and potential new targets for antitumor drugs.It has also been reported that Dip2 b is related to metabolism of fatty acids and its protein domain involved in metabolism of acyl coenzyme.The study of Dip2b's role in fatty acid metabolism and adipose tissue differentiation is of great importance for treatment of obesity and associated diseases.This study consists of two parts.In the first part,B16 melanoma cell was injected into wild type and Dip2b-LacZ(Heterozygous knockout of Dip2 b gene)mice subcutaneously,a tumor-burdened mouse model,to observe the tumor growth.H&E staining of tumor tissues,tumor size and weight measurement,and quantitative Real-time PCR were performed to analyze the tumor histology and gene expression.Results show that the subcutaneous growth rate of B16 cells in Dip2b-lacz mice was faster than that of wild-type mice,the tumor size was also larger than that of wild-type mice.Expression of tumor growth related genes was consistent with the phenotype.In summary,Dip2 b expression affects the tumorigenesis of B16 carcinoma.In the second part of this study,adipose tissues of 3-month-old Dip2b-lacZ and wild-type mice were analyzed for morphology by H&E staining and gene expression by quantitative real time PCR.Dip2b-lacZ mice were found to have brown fat whitening and white fat cells were smaller than the wild type,and some mice showed slight browning in white fat.VEGF and VEGFB expression were changed.Expression levels of adipose differentiation related factor mRNA were also altered.And the metabolic rate of Dip2b-LacZ mice was faster than that of WT mice.In conclusion,Dip2 b expression regulates tumor growth and participates in adipose differentiation and energy metabolism.Further study is needed to find out the regulatory mechanis.