The Gene Expression Profiles Of Visceral Adipose Tissue In Metabolic Syndrome And The Mechanism Of Metabolic Syndrome

Objective To compare the mRNA expression profiles of adipose tissues from patients with metabolic syndrome with the healthy controls and to explore the possible roles of some candidate genes in the pathogenesis of metabolic syndrome.Method1.The differentially expressed mRNA between MS and controls were studied by cDNA microarray, and confirmed by RT-PCR;2.By using db/db mice as metabolic syndrome animal model, verify the expression of differentially expressed genes in their epididymal adipose tissue.Results1.Compared with the normal control sample, the expression level of IL-1β, NR4A2and NR4A3in MS patient adipose tissue were significantly elevated (P<0.05), among which the NR4A2increased most significantly (P<0.01);2.Compared with the wild type, db/db mice have the elevated blood glucose, body weight and epididymal fat mass, as well as the expression of IL-1β, NR4A2and NR4A3in the epididymal adipose tissue (P<0.05), among which NR4A2increased most significantly (P<0.01).Conclusions In visceral adipose tissue of patients with metabolic syndrome, inflammatory factor IL-1β participates in the pathogenesis of metabolic syndrome; NR4A receptors play as regulators of lipid homeostasis.