Research Of Serum Exosomal MiRNAs In The Diagnosis Of Acute Cerebral Infarction

Objective To examine the concentration of serum exosomes and the expression levels of two brain-specific miRNAs(miR-9 and miR-124)in acute ischemic stroke(AIS)patients and to investigate the predictive value of the miRNAs for the diagnosis of AIS and assessment of injury.Methods1.Our study involved 65 AIS patients admitted to hospital from September 2014 to August 2015(onset time <72 hours)and 66 non-stroke volunteers examined in our hospital during the same time.We use ExoQuick kit to extract exosomes which were identified by transmission electron microscopy,nanoparticle tracking analysis and Western blot.Serum exosomal miR-9 and miR-124 expression levels were determined by real-time quantitative PCR.2.The diagnostic value of serum exosomal miR-9 and miR-124 for AIS was analyzed by ROC curve.Results1.The expression of miR-9 and miR-124 in serum exosomes of AIS was significantly increased compared with the control group(p<0.01).2.The expression of miR-9 and miR-124 in serum exosomes of AIS was positively correlated with the NIHSS score,infarct volume,and serum inflammatory factor IL-6(p<0.01).3.The areas under the ROC curve for serum exosomes miR-9 and miR-124 were 0.8026 and 0.6976.Conclusion The results show that serum exosomal miR-9 and miR-124 are potential biomarkers for diagnosing AIS and evaluating the degree of ischemic injury and provide novel ideas for exploring the prognosis of AIS patients.