QDPR Attenuates Renal Fibrosis By Regulating G?i2 Expression In UUO-induced Rats

Objectives Construction of a recombinant lentivirus with high expression of quinone dihydropteridine reductase(QDPR)and retrograde injected into the renal pelvis of fibrotic rats to observe its effect on G protein alpha inhibitory peptide 2(G?i2)and its possible mechanism in renal fibrosis.Methods Select 3 weeks of the size of the 12,SPF male SD rats randomly divided them into 4 groups:(1)UUO group,(2)Sham group,(3)control group,(4)QDPR high expression group(QDPR+).Rats in each group were sacrificed for anle 14 days after the operation.Renal tissue of the left obstructed rats was collected and the expression levels of QDPR,?-SMA,G?i2 protein and m RNA in each group were detected by immunohistochemical staining,western-blot and real-time fluorescence quantitative PCR(RT-PCR).Van Gieson(VG)staining was used to detect the changes of collagen deposition in the kidney of rats.Results 1 Animal models of rat renal interstitial fibrosis were successfully constructed: VG detected the collagen fiber deposition in the kidney of UUO group was significantly higher than that of Sham group(p < 0.05).Western-blot and RT-PCR results showed that the expression levels of QDPR protein and m RNA in the kidney of UUO group were significantly lower than those of Sham group(p < 0.05).The expression levels of ?-SMA,G?i2 protein and m RNA in UUO group were significantly upregulated compared with Sham group(p < 0.05).2 Successful build rat kidney high QDPR protein expression in animal models: QDPR high expression group VG staining results indicate a high degree of collagen deposition of significantly lower than that of control group(p < 0.05),and in group QDPR high expression of ?-SMA protein and m RNA expression is lower than the control group(p< 0.05),the expression of G?i2 protein and m RNA in QDPR high expression group was lower than that in control group(p < 0.05).Conclusions 1 In UUO model group,the expression level of QDPR was significantly down-regulated with renal tubular fibrosis in rats,suggesting that QDPR may play an important role in the progression of renal tubular fibrosis in rats.2 The high expression of QDPR significantly inhibited the expression level of G?i2,suggesting that the high expression of QDPR could play a protective role in the occurrence and development of uuo-induced renal interstitial fibrosis in rats by inhibiting the expression level of G?i2.Figure9;Table6;Reference 190...