| Objective: Some studies have confirmed that SAM-and SH3-domain containing 1(SASH1)as a new tumor suppressor gene is closely related to the occurrence,development and metastasis of many tumors.However,the mechanism by which SASH1 regulates breast cancer metastasis is not clear.This article aims to explore the molecular mechanism of the SASH1 which serves as a novel tumor suppressor gene to regulate breast cancer metastasis.Methods: 80 cases of breast cancer were collected from May 2015 to June 2016 in Chongqing Cancer Hospital.All cases were confirmed by HE staining.The expressions of SASH1,IQ motif-containing GTPase activating protein 1(IQGAP1)and E-cadherin in breast cancer tissues were analyzed by immunohistochemistry(IHC).The correlation among SASH1,IQGAP1 and E-cadherin,as well as the association of SASH1 and IQGAP1 expressions with the clinical parameters of breast cancer patients were analyzed,respectively.The recombinant plasmids HAIQGAP1-pcDNA3.0 and pEGFP-C3-SASH1 were cloned and transfected into human embryonic kidney HEK-293 T cells.The interaction of SASH1 and IQGAP1 was analyzed by immunoprecipitation-Western blotting.Results: In breast cancer tissues,there was a correlation between the expressions of SASH1 and IQGAP1(P<0.05),and the expressions of SASH1 and IQGAP1 proteins were respectively correlated with the expression of E-cadherin(both P<0.001).In addition,the expressions of SASH1 and IQGAP1 proteins were correlated with tumor diameter and tumor grade(all P<0.05),but without lymph node metastasis(both P>0.05).The recombinant plasmids HAIQGAP1-pcDNA3.0 and pEGFP-C3-SASH1 were constructed successfully.After these recombinant plasmids were transfected into HEK-293 T cells,the interaction between SASH1 and IQGAP1 was found.Conclusion: SASH1 interacts with IQGAP1,and which is closely related to the expression of E-cadherin.Therefore,it is suggested that SASH1 may form a new signaling cascade with IQGAP1 and E-cadherin to regulate breast cancer metastasis. |
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