The Expression And Clinical Significance KLF8?Snail1 And MMP9 Protein In Colon Cancer

Colon cancer is a kind of prevailing malignancy.Compared with some western countries,such as The United States,the age of colon cancer patients has been yong,which of the median age is around 45 years old,and the mortality of colon cancer patients in China has increased significantly in the past 30 years^[1].Colon cancer cells are easy to spread to distant areas,which is the main cause of tumor progression..Therefore,trying to explore the expression of related factors in the development and progression of colon cancer actively,and to find the therapeutic targets of the relevant factors are great significant for the study of clinical diagnosis and treatment of colon cancer.The main feature of epithelial-mesenchymal transition?EMT?is that the epithelial cells lose their polarity while obtaining interstitial properties,so that normal cells can be developed into malignant tumor cells.The normal cells even could obtain invasive ability to be metastasis.Some studies proved that KLF8 induces the development of epithelial-mesenchymal transition ?EMT?by down-regulating the expression of E-cadherin and further promotes the distant metastasis of tumor cells.Studies showed that Snail1can regulate down the expression of E-cadherin,which participates in the occurrence of EMT by changing the cell phenotype of cancer cells,reducing the adhesion between cells,and then enhancing the invasion and metastasis of cancer cells.Inducing disruption of the cell basement membrane barrier.Studies have shown that metalloproteinase-9?MMP9?can destroy the barrier of the cell membrane itself in tumor cells,and the loss of barrier function leads to cell infiltration and enhanced migration ability.At the same time,MMP9 can participate in the process of angiogenesis.The metastasis of tumor depends mainly on the continuous generation of new blood vessels.Therefore,the expression of MMP9 makes tumor cells more easily transfer to surrounding normal tissues,thus resulting tumor metastasis.Objective:By detecting the expression of KLF8,Snail1 and MMP9 in the selected colon cancer tissues and normal tissues surrounding colon cancer tissues,the expression of these three factors in colon cancer tissues and some clinicopathological features of patients were analyzed.The relationship between the two provides a new basis for the prognosis evaluation of cancer patients,and provides new targets for the development of therapeutic drugs for cancer patients.Methods:A total of 90 specimens of surgically resected and pathologically confirmed primary colon adenocarcinoma wax specimens and 30 specimens of normal tissue wax specimens around the colon cancer were collected,and all specimens were not included in the radiotherapy,chemotherapy,targeted therapy and immunotherapy and other treatmentsbefore surgery.The levels of KLF8,Snail1 and MMP9 in colon cancer tissues were detected by immunohistochemistry.The gender and age of the patients and tumor patients,the size of the tumor,the TNM stage of the patients,the depth of invasion of the tumor in the tissues and the presence or absence of lymph node metastasis were analyzed.The data were analyzed by SPSS 19.0software.The?2 test was used to count the data.The correlation between the three was judged by Spearman correlation analysis.The test standard was?=0.05,and P<0.05 was used as the difference.Results:Immunohistochemistry results1.KLF8,Snail1 and MMP9 were expressed in colon cancer tissues and adjacent normal colon tissues.The difference was that the expression of the three in colon cancer tissues was higher than that in normal tissues.KLF8and Snail1 were mainly expressed in cytoplasm,and the positive expression rates in cancer tissues were 62.2%?56/90?and 64.4%?58/90?,respectively,which were significantly higher than the positive expression rate of36.7%?11/30?in normal tissues adjacent to the cancerand 40.0%?12/30?,the difference was statistically significant??2=5.959,P<0.05;?2=5.531,P<0.05?.MMP9 was mainly expressed in the nucleus,and the positive rate in colon cancer tissues was 60.0%?54/90?,which was significantly higher than that in normal colon tissues?30.0%?9/30??.The difference was statistically significant??2=8.120,P<0.05?.The expression of KLF8,Snail1 and MMP9 in colon cancer was not related to gender,age,tumor size and tumor differentiation.There was no significant difference between the two groups?P>0.05?,and TNM stage of tumor.There was a correlation between the depth of invasion and the presence or absence of lymph node metastasis in the tissues,and the difference was statistically significant?P<0.05?.3.There is no correlation between KLF8 and Snail1 expression in colon cancer tissues?r=-0.173,P>0.05?;the expression of MMP9 was positively correlated with KLF8 and Snail1?r=0.346,P<0.05;r=0.199,P<0.05?,the difference was statistically significant?P<0.05?.Conclusion:The positive expression rates of KLF8,Snail1 and MMP9 in colon cancer tissues were significantly higher than those in adjacent normal tissues.The expression of KLF8,Snail1 and MMP9 in colon cancer tissues was consistent with the clinical TNM stage of tumors,The depth of invasion of the tumor in the tissue and the presence or absence of lymph node metastasis.The expression of MMP9 was positively correlated with KLF8 and Snail1,suggesting that the expression of KLF8,Snail1 and MMP9 in colon cancer can provide an important basis for the diagnosis and treatment of colon cancer.Provide important clues for the development of new targets for drugs.