| Objective:This study aimed to screen the SNP loci and haplotypes closely related to acute liver failure caused by HBV infection from the HMGB1 gene,and to conduct a correlation study,which may provide a new prediction method based on genes.It can predict the susceptibility of acute liver failure caused by HBV infection and provide early warning information for clinicians.Methods:Seven SNP loci were identified through systematic scanning of HMGB1 gene and searching a large number of related literature at home and abroad.SNP genotyping data of Han population in Beijing were downloaded from 1000 Genomes database.Linkage imbalance analysis was carried out by Haploview software to construct the haplotype ofthe gene and screen the labeled SNPs.From September 2012 to December 2018,64 patients with acute liver failure,33 males and 31 females,with an average age of(39.14± 8.28),were selected.The samples met the inclusion criteria of this subject.The informed consent of the subjects was required to collect the samples.The genotypes of SNPs in the disease group were genotyped by one-generation sequencing.The distribution differences of genotypes,alleles and haplotypes of labeled SNPs were analyzed by SPSS19.0 software.Results:Five common haplotypes(C-C,T-G,G-T-A,G-C-G-C,C-C-G-C)and three labeled SNPs(rs1045411,rs2249825,rs1412125)were constructed by searching for HMGB1 gene on human chromosome 13.The distribution of genotype and allele frequencies of rs2249825 and rs1412125 in disease group and control group was statistically different(P < 0.05).The frequencies of G-C-G-C and C-C-G-C constructed by rs2249825,rs1412125,rs9508752 and rs2892547 loci were significantly different between the two groups(P < 0.05).Conclusion:The polymorphism of HMGB1 gene was significantly correlated with acute liver failure caused by HBV infection.The haplotypes constructed included G-C-G-C and C-C-G-C(rs2249825-rs1412125-rs9508752-rs2892547),and the selected labels included rs2249825 and rs1412125.These haplotypes may increase the risk of acute liver failure caused by HBV infection,and the selected population labeled with SNPs carrying C allele may also increase the susceptibility to acute liver failure caused by HBV infection. |
