Study On The Functional Components Of Acanthopanax Sentionsus For Improving Brain Danage In Irradiated Mouse

Space radiation can cause damage to the nervous system.The functional components of Acanthopanax senticosus（AS）have therapeutic effects on nervous system diseases.It was important to study the functional components of AS on the improvement of radiation-induced brain injury diseases.the main active components in AS,including Acanthopanax senticosus polysaccharide（ASPS）,flavones,syringin,and eleutheroside E（EE）was studied,the mouse brain injury model irradiated by ⁶⁰Co-γray as a research perspective.Through the study on the effects of AS functional components on learning and memory ability,the improvement of oxidative damage and the metabolism in brain tissue of radiation-induced brain injury mouse,the improvement effects of different AS functional components on radiation-induced brain injury mouse were explored,and the results as follows:Firstly,the effects of AS functional components on improving learning and memory ability of radiation-induced brain injury mice were investigated through behavioral test,pathological section observation,and neurotransmitter content determination.In behavioral experiments,the water maze test index of syringin and EE was significantly improved compared with the radiation group（P<0.01）,which indicated that syringin and EE could improve the spatial memory ability and nerve sensitivity of irradiated mouse.In the pathological observation of cerebral cortex and hippocampal tissue of irradiated mouse,it was found that EE had small cell cavities,abundant nerve cells,indicating that EE could improve the damage of brain tissue cells caused by radiation.In the determination of neurotransmitter content,ASPS could significantly increase the content of norepinephrine（NE）in irradiated mouse brain tissue（P<0.01）.Flavones could significantly increase the content of norepinephrine（NE）and acetylcholine.（Ach）（P<0.05）,Syringin and EE could significantly increase the content ofγ-aminobutyric acid（GABA）（P<0.05）and acetylcholine（P<0.05）.The results indicated that ASPS could play a role in NE energy system,flavones could play a role in NE energy system and Ach energy system.Syringin and EE could improve the learning and memory ability of radiated mice by acting on GABA system and Ach system.Then the improvement of immune injury and oxidative injury of AS components on irradiated mouse was explored through the chest and spleen index and the oxidative index of brain tissue and serum in irradiated mouse.It was found that the components of AS could significantly improve radiation-induced immune injury.In brain tissue,the ASPS and flavones could significantly reduce the content of carbonyl protein and reduce the content of free radicals,indicating that ASPS and flavones could improve the denaturation of protein caused by radiation,scavenge free radicals and prevent lipid peroxidation,but there was no significant differences between syringin and group in the index of SOD and CAT,and EE on glutathione（GSH）was not obvious.In serum,the components of AS could inhibit radiation biological macromolecular damage,ASPS and syringin could play a significant effect on scavenging free radical free radicals.The effect of flavones on superoxide dismutase（SOD）and catalase（CAT）was not significant,and EE has no significant effect on GSH compared with the model group.It indicated that ASPS and flavones in brain tissue play a role in inhibiting protein damage and scavenging free radicals,ASPS and syringin in serum make effects on inhibiting biological macromolecular damage and scavenging free radicals.Finally,through the pharmacokinetics and mass spectrometry analysis of the functional components of AS in brain and other tissues,explored the metabolism of AS in the brain and other tissues of mice and the components of the blood-brain barrier.The results showed that the functional components of AS could play a role in various tissues.The metabolism of EE in brain tissue was significantly higher than that in other tissues,indicating that Acanthopanax Saponin E could play a role in brain tissue.The pharmacokinetic results showed that the pharmacokinetic curve of flavones showed double peaks,indicating that the drug redistribution of flavones occurred in brain tissue.The drug clearance and bioavailability of EE were significantly higher than other components,indicating that EE could be absorbed in the brain tissue of irradiated mouse,and was removed in time after drug action;By mass spectrometric analysis of brain tissue of irradiated mouse,the intact compound monomer detected was 2,3,4-tri-O-benzyl-L-furan pyran the polysaccharide monomer,hormone the flavonoid monomer,as well as isoxazinidine,α-linolenic acid,and eleutheroside E1,indicate that several monomers could pass through the blood-brain barrier and maintained the stability of the nervous system.