| Objective: By studying the effects of different doses of Zha Chong Shi San Wei PiLLs and its similar formula of decoction on the content of cTnI and NT-ProBNP in rat serum,and simultaneously detecting the content of aconitine in the drug-containing heart tissue,to exPlore the cardiotoxicity and toxicological basis of Zha Chong Shi San Wei PiLLs and its similar formula of decoction for experimental rats.To explore the myocardial toxicity and material basis of the Mongolian medicine Zha Chong Shi San Wei PiLLs and the same formula of the decoction of the same Prescription on the experimental rats.Therefore,it provides experimental data support for guiding the clinical safety medication of Mongolian medicine Zha Chong Shi San Wei PiLLs and the medical dispute for medical disputes caused by the drug causing myocardial poisoning.Methods: 50 healthy adult male SD rats,weighing 200±20 g,were housed in a constant temperature clean room for 2 weeks.According to the body weight,they were randomly divided into the blank group,the Zha Chong Shi San Wei PiLLs clinical dose group and the high dose group(Hereinafter referred to as the drug clinical dose group,the drug high dose group),and the clinical formula of the same formula with Zha Chong Shi San Wei PiLLs.The dose group and the high dose group(hereinafter referred to as the crude drug clinical dose group,the crude drug high dose group),a total of 5 groups,each group of 8;The rats in the treatment group were intragastrically administered with the corresponding dose of the drug,and the rats in the blank group were given the same amount of distilled water once a day for 28 days.On the 28 th day,the heart rate and electrocardiogram of each group were detected by BL-420E+ bio-functional test system.On the 29 th day,the rats were sacrificed after blood was taken from the abdominal aorta,and the organ coefficient was calculated,and a test sample was prepared.The contents of cTnI and NT-Pro BNP in serum of each group were detected by microplate reader.The pathological changes of myocardial tissue in each group were observed by HE staining.At the same time,the content of aconitine in myocardial tissue of each group was determined by ultra performance liquid chromatography.The SPSS 22.0 statistical software was used to analyze and compare the differences of various indicators in different groups.Results:(1)The rats in the clinical dose group were generally in good condition for 28 days.Compared with the blank group,the heart rate and electrocardiogram of the rats in the clinical dose group and the crude drug group were normal;there was no significant difference in organ coefficient(P > 0.05);There were no significant differences in cTnI and NT-ProBNP levels(P > 0.05).No abnormalities were observed in histopathological myocardial tissue.(2)Rats in the high-dose group showed adverse reactions such as dull skin color and decreased activity during the 28 days of treatment.Compared with the blank group,the heart rate of the high-dose group and the high-dose group were slowed down(P <0.05).The electrocardiogram showed abnormal manifestations such as arrhythmia;The organ coefficients were increased to different degrees(P <0.05),and the increase in the drug group was more significant(P <0.05).;The serum cTnI levels were increased in different degrees(P <0.05),and the serum cTnI content in the crude drug group was higher than that in the drug group(P <0.05).There was no significant difference in the serum levels of NT-Pro BNP between the high-dose group and the high-dose group(P>0.05).HistoPathological observation showed that there were mild edema,steatosis and other Pathological damages in rat cardiomyocytes,and the damage of the crude drug group was heavier than that of the drug group.(3)The results of aconitine content determination: the content of aconitine in the myocardial tissue of the high dose group was significantly higher than that of the clinical dose group(P <0.05).The content of aconitine in the myocardial tissue of the high dose group was higher than that of the traditional drug group(P <0.05).Conclusion:(1)The clinical dose of the Mongolian medicine Zha Chong Shi San Wei PiLLs and the crude drug decoction had no obvious toxic effects on the myocardium of experimental rats.(2)High-dose Mongolian medicine Zha Chong Shi San Wei PiLLs and crude drug decoction have certain toxic effects on the myocardium of experimental rats.The toxicity of crude drug decoction is more important than that of Mongolian medicine;and the content of aconitine in myocardial tissue is determined.The results showed that the content of aconitine in the myocardial tissue of the high-dose group was significantly higher than that of the clinical dose group.The content of aconitine in the myocardial tissue of the high-dose group of crude drug was higher than that of the Mongolian medicine Zha Chong Shi San Wei PiLLs group,it is believed that the myocardial toxicity of the Mongolian medicine Zha Chong Shi San Wei PiLLs,which was made by fully Processing the crude drug such as sage,may be related to the content of aconitine in myocardial tissue to some extent. |
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