The Study On Endometrial Receptivity Of The Obese PCOS Female Rats Model Through Sphk1/S1P-PI3K/AKT/CyclinD1 Pathway By Tonifying Kidney And Removing Phlegm

Objective: The purpose of this study was to observe the effect of tonifying kidney and removing phlegm(TKRP)through Sphk1/S1P-PI3K/AKT/CyclinD1 signal pathway on intimal receptivity in female rats with obese polycystic ovary syndrome(PCOS).Methods:The 7~8 week old SPF female SD rats were divided into the blank control group and the model group at 1:5.In the model group,the obese PCOS female model was established by intragastric administration of letrozole and high fat suspension for28 days.The model group was divided into the model control group,the high-dose group of the traditional Chinese medicine(TCM),the middle-dose group of the TCM,the low-dose group of the TCM,the positive control drug group at 1:1:1:1:1 after successful establishment of the model.The equivalent volume of the TCM was given to the high,middle and low dose group of the TCM,and the positive control group was given the same volume metformin solution and the blank control group and the model control group are respectively provided with equal volume of distilled water.After 21 days of intervention the TCM for TKRP,serum PROG,T content was detected by Elisa and The mRNA expression of Sphkl,S1PR1,PI3 K,CyclinD1,a molecule related to Sphk1/S1P-PI3K/AKT/CyclinD1 signaling pathway in uterus,was detected by Realtime-qPCR.Results:1.The effect of TKRP on the body mass growth rate of the obese PCOS model female rats: Compared with the blank control group,the body mass growth rate of obese PCOS female rats in the model control group was higher than that in the blank control group(P<0.05).Compared with the model control group,the increase of body mass in the positive drug group,the high dose group and the middle dose group decreased(P<0.05).2.The effect of TKRP on pregnancy rate of the obese PCOS model female rats:Compared with the blank control group,the number of pregnant the obese PCOS model female rats in the model control group decreased;Compared with the modelcontrol group,the number of pregnant the obese PCOS model female rats in the high dose group and the middle and low dose group increased,and the number of pregnancy in the high dose group was higher than that in the other TCM groups.3.The effect of TKRP on serum sex hormones in the obese PCOS model female rats: Compared with the blank control group,The concentration of serum PROG in the model control group was significantly lower than that in the control group(P<0.01),T concentration was significantly increased(P<0.01).Compared with the model control group,the concentration of PROG in serum of rats in positive drug group and high dose group of TCM was increased(P<0.05),T concentration decreased(P<0.05).4.The effect of TKRP on uterine Sphk1,S1PR1,PI3 K,CyclinD1 in the obese PCOS model female rats: Compared with the blank control group,the expression of Sphk1 mRNA,S1Pr1mRNA,PI3 KmRNA,CyclinD1mRNA in the uterus of the model control group was lower than that of the blank control group(P>0.05 or P<0.01).Compared with the model control group,the expression of Sphk1 mRNA,S1Pr1mRNA,PI3 KmRNA,CyclinD1mRNA in uterus of rats with high dose of tTCM was increased(P<0.05 ? P>0.05 ? P<0.01).Conclusion:1.The method of TKRP can inhibit the continuous growth of body mass in the obese PCOS model female rats,thus improving the obesity status of female rats with PCOS.2.The method of TKRP can improve the reproductive endocrine disorder of the obese PCOS model female rats.3.The method of TKRP may activate Sphk1/S1P-PI3K/AKT/CyclinD1 pathway by up-regulating Sphk1,S1PR1,PI3 K,CyclinD1,which can promote local uterine angiogenesis and improve ER,so as to improve the pregnancy rate of the obese PCOS model female rats.