S100A13 Protein Is Associated With Tumor Angiogenesis And Survival In Patients With Early NSCLC

Background and purpose: Lung cancer,one of the most common cancers in the world,has a complex disease mechanism with genetic and environmental factors,and the interactions between these factors contribute to the development of this fatal disease.The five-year survival rate of non-small cell lung cancer(NSCLC)(about 85% of lung cancer(LC)cases)is still very low.By far,the most promising strategy for improving progression-free survival and total survival(OS)in NSCLC patients is early diagnosis followed by surgical excision,which is currently the standard treatment.However,at the time of diagnosis most NSCLC patients have progressed to an advanced or metastatic stage that is inoperable.Despite advances in early detection and treatment,overall 5-year survival is low.Therefore,further studies on the mechanisms of initiation and progression,as well as the development of prognostic biomarkers and drug targets,are needed to improve the prognosis of NSCLC and allow for individualized treatment.Multiple S100 proteins have been implicated in the malregulated expression of many tumors.S100A13 is a small calcium(Ca 2+)binding protein belonging to the S100 family.S100A13 plays a key role in tumor growth and metastasis.The purpose of this study was to investigate the prognostic significance of S100A13 expression,microvascular density(MVD)and early non-small cell lung cancer(NSCLC)survival.Research methods: Machine analysis was performed from the GEO database to determine the association between S100A13 and NSCLC.Kaplan-Meier method was used to evaluate the data of 82 patients with early NSCLC undergoing radical resection,and the relevant survival curves were drawn.Tumor specimens were then paraffin-embedded using immunohistochemistry and stained with S100A13 and CD31(specific endothelial markers)to confirm the relationship between cancer cells and microvascular density.Univariate and multivariate analyses were used to evaluate the prognostic significance.Research result: Overexpression of S100A13 m RNA in NSCLC was analyzed by correlation database,especially in late stage.Of the 82 NSCLC samples examined,37(45.1%)showed S100A13 overexpression and 31(37.8%)showed high MVD.Univariate analysis showed that gender,age,smoking status,histological type,tumor differentiation and T stage were not significantly correlated with prognosis.However,overall and disease-free survival was significantly lower in patients with S100A13 overexpression and high MVD than in the rest of the patients.Multivariate analysis showed that only S100A13 overexpression was an independent factor for poor prognosis of early NSCLC.Statistical analysis showed that MVD was significantly higher in tumors with high S100A13 expression(67.6%,25/37)than in tumors with low S100A13 expression(13.3%,6/45)(P <0.01).Analysis conclusion: High S100A13 expression is closely related to high intratumor angiogenesis and poor prognosis of stage I NSCLC patients.The immunohistochemical evaluation of S100A13 expression and the examination of the perioperative degree of vascular and lymphatic invasion are valuable for predicting the prognosis.