Effects Of Vitamin D Deficiency On Glucolipid Metabolism,Morphology And Function Of Ovary In Female Rats

Objective To investigate the effects of vitamin D deficiency on glucolipid metabolism,morphology and function of ovary in female rats.Methods Forty-eight female Sprague-Dawley(SD)(3-week-old)rats were randomly divided into control group(n=24)and vitamin D deficiency group(n=24).All rats in control group were fed with standard diet and all rats in vitamin D deficiency group were fed with vitamin D deficient diet.On the 6th.14th and 22th week 8 rats from each group were randomly selected and received the estrus cycle by vaginal smears for 2 weeks,they were sacrificed on the 8th,16th,24th week and serum as well as ovary specimens were collected.Serum 25(OH)D,calcium,phosphorus,fasting blood glucose,fasting insulin,triglyceride,total cholesterol,testosterone,estrogen,progestogen,luteinizing hormone(LH),follicle stimulating hormone(FSH)and anti-Mullerian hormone were measured.Homeostatic model assessment-insulin resistance(HOMA-IR)and LH/FSH ratio were calculated.Ovarian morphologic changes were observed by HE staining.Results On the 16th,24th week,compared with control group,serum triglyceride,fasting blood glucose,fasting insulin,HOMA-IR,LH/FSH ratio,anti-Mullerian hormone levels were increased(t=-4.02?3.87,all P<0.05),while on the 24th week,compared with control group,the FSH level in vitamin D deficiency group was decreased(t=2.35,P<0.05),and ovarian polycystic alterations were observed in vitamin D deficiency group.No significant difference in serum total cholesterol,testosterone,estrogen,progestogen levels and the estrous cycle were found between the two groups(all P>0.05).Conclusions Vitamin D deficiency may affect glucolipid metabolism,morphology and function of ovary in female rats.