Role And Mechanism Research On Anti-Athletic Fatigue Of Total Saponins From Astragalus

Research purposes: From the perspective of sports human science,this paper demonstrates that total saponins of Astragalus membranaceus can improve the body's anti-sport fatigue and improve the body's endurance,and explore the application of the pharmacological effects of total saponins of Astragalus membranaceus in sports,so as to better serve sports.Training will improve the athlete's athletic ability.Total saponin of Astragalus membranaceus(TSA)is a main component extracted from the roots of Astragalus membranaceus.It has a conditioning effect on blood sugar in the body,can effectively enhance the body's immunity,promote its growth,and also has antioxidant capacity to the body.According to the relevant literature,this Chinese medicine ingredient also has a good anti-fatigue effect in inhibiting skeletal muscle contraction.research method:About 200 healthy bullfrogs were selected for the experimental animals,which were divided into several groups,and each group was quantified by twelve.First,the preparation of the frog sciatic nerve dry specimen was performed.In order to verify the effects of different concentrations of total saponins of Astragalus on the anti-exercise fatigue of skeletal muscle of animals,the concentration gradient of total saponins of Astragalus membranaceus was set to 0mg/L,10mg/L,15mg/L,50mg/L,100mg/L.Different doses of total saponin of Astragalus membranaceus were dissolved in Renshui(RS),and the contraction function intensity of frog skeletal muscle samples was collected using MD3000 instrument.The saturation stimulation intensity and threshold stimulation intensity of each nerve were found before each experiment.Repeated stimulation of saturated stimulation intensity was delivered at 1Hz,and the waveform was observed and recorded to achieve 50% amplitude and 10%amplitude time with reference to the maximum amplitude(100%)in the skeletal muscle contraction amplitude.The time to reach 50% and 10% of the maximum amplitude(initial value)is marked as T50% and T10%,respectively.When the muscle contraction force is reduced to 10% of the maximum amplitude(initial value)induced by the anterior contraction force,it is regarded as fatigue.For the post-exercise contraction force recovery test,when the muscle contractility decreased to 10% of the maximum amplitude(initial value)caused by the previous,the stimulation was repeated using the same stimulation intensity pulse every 30 seconds to 10 minutes.Muscle recovery was analyzed using the last single muscle contraction amplitude at the 10 th minute.The 60% shrinkage amplitude recovery of the maximum amplitude(initial value)is set to the acceptable recovery data threshold.In order to avoid tissue death,the stimulus was kept constant until the muscle contraction tension dropped to 10% of the original record.Collect all the data.On the basis of the above experiments,based on the 50mg/L total saponin solution of Astragalus membranaceus,the RS+CdCl2 experimental group and the RS+LaCl3 experimental group with the voltage-gated calcium ion broad-spectrum inhibitor were used.The experimental group was used to record the latency and amplitude changes of the sciatic nerve complex action potential in the basic experimental group and the 50 mg/L total saponin solution.The data of the latency and amplitude of the action potential of the sciatic nerve in the dosing and non-administered groups were compared between the normal RS+CdCl2 treatment and the RS+LaCl3 treatment.Experimental results:1.The skeletal muscle contraction intensity was significantly changed by the concentration of total saponin solution in the time of 50% of the maximum amplitude(initial value)(P<0.05 for each concentration and blank control group).The effect is remarkable.2.There was no significant effect on the skeletal muscle contraction tension to10% of the maximum amplitude(initial value)at all TSA concentrations(P>0.05).Changes in the concentration of STA solution at 0-10 mg/L,0-25 mg/L,0-50 mg/L,0-100 mg/L did not cause a significant change in skeletal muscle fatigue resistance.When the skeletal muscle contraction tension is reduced to 10% of the maximum amplitude(initial value),the skeletal muscle has severe exercise fatigue,or has lost mobility.At this time,the effect of STA solution on it is weak and negligible.3.The recovery rate of skeletal muscle anti-exercise fatigue measured at different TSA concentrations increased with increasing concentration(experimentalmeasured concentrations compared with blank control group,P<0.05)4.After the administration of the normal RS treatment group and the data of the action potential of the sciatic nerve before administration,it was found that the potential waveform after administration showed a significant change: the duration of the action potential latency increased significantly,and the action potential amplitude increased.The results indicate that total saponins of Astragalus membranaceus can inhibit the conduction velocity of action potential of sciatic nerve trunk and enhance the excitability of sciatic nerve trunk.5.In the RS+CdCl2 blocker treatment group and the RS+LaCl3 blocker treatment group,the data of the sciatic nerve action potential before administration and the pre-dose sciatic nerve action potential showed no significant difference in the post-dose potential waveform,action potential There was no significant change in latency and magnitude.in conclusion:Total saponins of Astragalus membranaceus have a good effect on inhibiting skeletal muscle contraction and are related to the dependence of calcium ion.The effect of total saponins of Astragalus on the action potential of sciatic nerve is achieved by activating voltage-gated calcium channels.