| ObjectiveEstablishment of animal model of exercise fatigue, observe the morphological structure of liver, serum ALT, AST content in the evaluation of liver injury from hepatic protective effect, free radical, lactic acid and glycogen metabolism, and apoptosis of liver cells to start, to explore themechanism of acupoint injection of total saponins of Panax ginseng on liver in rats with exercise induced fatigue.Methods24 rats were randomly divided into blank group (0.2 mL saline acupoint injection), exercise group of 21 days (0.2 mL saline acupoint injection) and total saponins of Panax ginseng acupoint injection group (0.2 mL of total saponin of Panax ginseng acupoint injection) group of 3. Exceptthe rats in the blank group (normal diet), the rest of the group with high 60cm,55cm diameterplastic drums as swimming rats groove depth of 50cm, the water temperature of 33 plus or minus 2 DEG C, indications of training for 2 days, began formal experiments to test for. third days. Every acupoint injection (unilateral, Zusanli and Neiguan, alternating every other day) were administered once injection after 1 hours, except for the rats in the blank group, the other ratsswimming training 2 times a day, each time the free swimming 15min, gap 10min, training for 21days in a row. In order to ensure the quality of swimming training, ready to stick in the barreldrive grilled pool wall edge or other rats, the rest of the rat. In order to ensure the safety of rattraining, and when the rats were exhausted, then stop the swimming training. In the lastmovement, participating in exercise training rats completed the last exercise training after 2h, the rats were killed:the separation of blood, serum,-80 C preservation, for the determination ofalanine aminotransferase (ALT) and aspartate aminotransferase (AST) content; the left lobe of the liver tissue section: take right amount (the same site), the temperature of -80 DEG C frozenfor the determination of liver total antioxidant capacity (T-AOC), hepatic malondialdehyde (MDA), lactic acid (LA), nitric oxide (NO) and glucose (Glu) content in liver lactate dehydrogenase (LDH), inducible nitric oxide synthase (iNOS), Caspase-3 and Caspase-8 activity. Takes the right lobe of the liver tissue amount (in the same position), soaked in 10% formalin fixed for 24 h, HE staining, light microscope to observe the pathological changes in liver tissues.Results1. rats in the blank group the hepatic lobule structure is normal, periportal morphological integrity, no degeneration and necrosis of liver cells, a single nuclear membrane integrity, clear nucleus, were round or oval, intracellular visible small lipid droplets, mitochondria of normal morphology, cristae structure integrity, rough endoplasmic reticulum arranged uniformly and liver cell model;rats appeared vacuolization, ballooning degeneration, scattered punctate, piecemeal necrosis and inflammatory cell infiltration; acupoint injection group the degree of liver tissuelesion of rats with relatively light, between the blank group and the model group.2. compared with the blank group, the model group and the acupuncture point injection grouprats serum ALT and AST content were significantly increased (all p<0.01); compared with the model group, acupoint injection group rats serum ALT and AST contents were significantlydecreased (all p<0.01).3. compared with the blank group, the model group and the acupuncture point injection group of rat liver T-AOC activity and MDA content were significantly increased (all p<0.01); compared with model group, acupoint injection of rat liver T-AOC activity and MDA content were significantlydecreased (all p<0.01).4. compared with the control group, the hepatic content of lactic acid and lactate dehydrogenase activity in the rats of model group were significantly increased, the content of liver glycogenincreased (all p<0.01), hepatic lactate content in acupoint injection group rats increased significantly (p<0.01), but the liver lactate dehydrogenase activity and glycogen content showed no significant difference (both p>0.05); compared with the model group, acupoint injection groupof rat liver lactate content and lactate dehydrogenase activity was significantly decreased (all p<0.01), the content of liver glycogen increased significantly (p<0.01).5. compared with the control group, the model group and the acupuncture point injection groupof rat liver NO content, iNOS, caspase-3 and caspase-8 activity were significantly increased (all p<0.01); compared with model group, the NO content in liver of acupoint injection in rats, iNOS, caspase-3 and caspase-8 activities were significantly lowered (both p<0.01).Conclusion:1. in order to "high 60cm, diameter 55cm plastic drums as rat swimming tank,50cm water depth, water temperature 33-2 DEG C, the adult SD male rats swimming training every day 2 times, each time the free swimming 15min, 10min clearance, training for 21 days in a row, in theswimming training in rats, with a stick in the drive Ba the barrel wall of the pool edge or otherrats, the rest of the rat, and when rats were exhausted, then stop the swimming training" as themodeling methods, can copy the successful exercise fatigue animal (rat) model.2. Zusanli and Neiguan alternating every other day, unilateral, acupoint injection of ginseng saponins, can achieve the anti fatigue effect, can make the exercise-induced fatigue rats serum ALT and AST contents were significantly decreased after HE staining of rat liver lobule visible, periportal form more complete, degeneration and necrosis of liver cells less, single membrane, nucleus and cytoplasm structure relatively intact rat liver tissue lesions can be effectively improved.3. mechanisms of acupoint injection of total saponin of Panax ginseng on liver in rats with exercise induced fatigue may be correct, abnormal hepatic glycogen and lactate metabolism, reduce liver free radical injury, downregulation of iNOS, adjust the NO quantity, activation oftermination of Caspase-3 and caspase-8, apoptosis of liver cells.Results1. rats in the blank group the hepatic lobule structure is normal, periportal morphological integrity, no degeneration and necrosis of liver cells, a single nuclear membrane integrity, clear nucleus, were round or oval, intracellular visible small lipid droplets, mitochondria cristae normal morphology, structural integrity, rough endoplasmic reticulum arranged uniformly and liver cell model; rats appeared vacuolization, ballooning degeneration, scattered in the spot, piecemeal necrosis and inflammatory cell infiltration; acupoint injection group the degree of liver tissuelesion of rats with relatively light, between the blank group and the model group.2. compared with the blank group, the model group and the acupuncture point injection grouprats serum ALT, AST content were significantly increased (all p<0.01); compared with the model group, acupoint injection group rats serum ALT, AST levels were significantly reduced (both p<0.01).3. compared with the control group, the model group and the acupuncture point injection groupof rat liver T-AOC activity, MDA content were significantly increased (all p<0.01); compared with model group, acupoint injection of rat liver T-AOC activity, MDA content were significantlydecreased (all p<0.01).4. compared with the control group, the hepatic content of lactic acid and lactate dehydrogenase activity in the rats of model group were significantly increased, the content of liver glycogenincreased (all p<0.01), hepatic lactate content in acupoint injection group rats increased significantly (p<0.01), but the liver lactate dehydrogenase activity and glycogen content showed no significant difference (both p>0.05); compared with the model group, acupoint injection groupof rat liver lactate content and lactate dehydrogenase activity was significantly decreased (all p<0.01), the content of liver glycogen increased significantly (P<0.01).5. compared with the control group, the model group and the acupuncture point injection groupof rat liver NO content, iNOS, caspase-3 and caspase-8 activity were significantly increased (all p<0.01); compared with model group, the NO content in liver of acupoint injection in rats, iNOS, caspase-3 and caspase-8 activities were significantly lowered (both p<0.01).Conclusion1. in order to "high 60cm, diameter 55cm plastic drums as rat swimming tank,50cm water depth, water temperature 33-2 DEG C, the adult SD male rats swimming training every day 2 times, each time the free swimming 15min, 10min clearance, training for 21 days in a row, in theswimming training in rats, with a stick in the drive Ba the barrel wall of the pool edge or otherrats, the rest of the rat, and when rats were exhausted, then stop the swimming training" as themodeling methods, can copy the successful exercise fatigue animal (rat) model.2. Zusanli and Neiguan alternating every other day, unilateral, acupoint injection of ginseng saponins, can achieve the anti fatigue effect, can make the exercise-induced fatigue rats serum ALT, AST levels were significantly reduced, after HE staining of rat liver lobules, visible, periportal form more complete, degeneration and necrosis of liver cells less, single membrane, cytoplasmic and nuclear structure relatively intact, rat liver tissue lesions can be effectively improved.3. mechanisms of acupoint injection of total saponin of Panax ginseng on liver in rats with exercise induced fatigue may be correct, abnormal hepatic glycogen and lactate metabolism, reduce liver free radical injury, downregulation of iNOS, adjust the NO quantity, activation oftermination of Caspase-3 and caspase-8, apoptosis of liver cells. |
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