| Purpose:This project aims to provide a reference for the clinical application of semi-implanted electrical stimulation by constructing a rabbit model of acute sciatic nerve injury and studying the effect of semi-implanted electrical stimulation on peripheral nerve injury.Methods:First,32 experimental male rabbits were randomly selected to establish an acute sciatic nerve injury model.One week after the model was successfully established,it was randomly divided into two groups:16 in the experimental group and 16 in the control group.The left side was the experimental side and the contralateral self-control was performed at the same time.An electrode guide wire is placed proximal to the injured suture of the epineurium epithelium,and an electrode wire is embedded in the distal muscle tissue.The electrode passes through the subcutaneous tunnel and leads out of the skin.The next electrode line is connected to the semi-implanted Electrical stimulation device,the experimental rabbits were subjected to 4hours of daily electrical stimulation(stimulation parameters:frequency:20hz;pulse width:100us;voltage:9V;wave type:square wave).At the third and sixth weekends after surgery,materials were obtained to calculate the sacral nerve conduction velocity,the recovery rate of wet weight of gastrocnemius muscle,the cross-sectional area of muscle fiber,the pathological section of gastrocnemius muscle and the pathological section of sciatic nerve.Statistical analysis of experimental data.Results:1.General conditions:All 32 rabbits were successful in surgery,and none died.At the third and sixth weekends after the removal of the electrode guide wire,the guide wire showed no obvious change in color,no traces of fracture and corrosion,no obvious fluid infiltration,no ulceration and necrosis in the muscles and skin.2.At the end of the third postoperative week,electromyographic data were recorded to calculate the phrenic nerve conduction velocity:104.83±1.25 m/s in the experimental group and 84.31±1.13 m/s in the control group.P<0.05 was considered statistically significant,indicating significant differences between the two groups.At the end of the sixth week after surgery,the experimental group was 107.29±2.12 m/s and the control group was55.37±1.24 m/s,P<0.01 was statistically significant,indicating that the difference between the two groups was significant.3.At the end of the third postoperative week,the recovery rate of wet weight of rabbit gastrocnemius muscle was calculated as:78.34±6.51%in the experimental group and 59.23±4.68%in the control group,P<0.05 was statistically significant,indicating a significant difference between the two groups;The experimental group was 89.63±6.57%and the control group was44.16±3.24%,P<0.01 was statistically significant,indicating that the difference between the two groups was very significant.4.Histological findings:(1)HE staining histological observation of gastrocnemius at the third weekend and sixth weekend showed that muscle fibers of experimental group and control group had different degrees of atrophy and increased cell gaps at the third weekend postoperatively.The corresponding degree of the experimental group was significantly lighter than that of the control group,and the difference between the experimental group and the control group was significant.At the end of the sixth week after surgery,the degree of muscle fiber atrophy in the experimental group was improved compared with three weeks ago.However,muscle fibers in the control group were more atrophic than those in the three weeks before,and the intercellular space was increased compared with three weeks ago.The arrangement of muscle fiber cells was disordered,and the difference between the experimental group and the control group was significant.At the third weekend postoperatively,the results of calculation of myofiber cell cross-sectional area were:experimental group 4768.27±263.17um~2,control group 3632.33±203.63um~2,P<0.05 was statistically significant,indicating a significant difference between the two groups:postoperative sixth weekend,the experiment Group 5024.13±273.25um~2,control group3489.15±263.27um~2,P<0.05 was statistically significant,indicating significant difference between the two groups(2)HE staining of the sciatic nerve of the experimental group and the control group at the third weekend and the sixth weekend after surgery.In the experimental group,the axons were thick and the structure of the myelin sheath was normal,while in the control group,the myelin sheaths of the control group had varying degrees of degeneration,relaxation,and bending,and there was a significant difference between the experimental group and the control group.Conclusion:Semi-implanted electrical stimulation can promote the growth rate of nerve regeneration,and is conducive to recovery of muscle wet weight.Its curative effect on denervation-induced muscle atrophy is definite;it may become the main means of rehabilitation therapy after peripheral nerve injury in clinical practice. |
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