The Study On The Effects Of Bushen Zhuangdu Recipe On The Expression Of Bax And Cytochrome-c In In Apoptotic Nucleus Pulposus Cells

Objective:To treat the nucleus pulposus cells with hydrogen peroxide in vitro,and to induce the apoptosis model of nucleus pulposus cells,and to observe the effect of Bushen Zhuangdu Recipe on the expression of mitochondria-dependent cells in the nucleus pulposus cells to regulate the expression of Bax and cyt-c.The mechanism of Bushen Zhuangdu recipe delaying the apoptosis of nucleus pulposus cellssMethod:1.Model preparation experiment:Primary nucleus pulposus cells were cultured in vitro to the third generation,followed by modeling with hydrogen peroxide,Then use TUNEL to detect apoptosis detection.2.37 male rats were divided into three groups with random numbers,10 in each group,and the remaining material was blank.After the stomach was administered with the Chinese medicine suspension 10 days,blood was taken from the abdominal aorta to prepare the drug-containing serum.3.Cultured nuclous pulposus cells were cultured woth drug-containing serum,cultured,and then the content of Bax and cyt-c rotein and mRNA in the cells were detected by WB and PCR.Result:The comparison between the apoptosis index of each group showed that there were statistical differences in the apoptotie index between the low concentration,medium concentration and high concentration groups compared with the model group(P<0.05).There were differences in the expression of Bax and cyt-c protin and mRNA in the low,medium and high concentrations of the model group(P<0.05),but compared with the Bax and cyt-c proteins and mRNA in the cells.The score was statistically significant(P<0.05).Conclusion:1.In the model of medullary cell apoptosis induced by Chinese medicine,the apoptosis of nucleus pulposus cells was positively correlated with the expression of proapoptotic protein Bax and cyt-c;it was confirmed that mitochondrial pathway plays an important role in the apoptosis process of nucleus pulposus cells.2.Bushen Zhuangdu can inhibit the expression of Bax and cyt-C protein in nucleus pulposus cells,thereby delaying IDD,and its mechanism of action is related to inhibition of mitochondria-mediated apoptosis pathway and dose-related at a certain concentration.