Phagocytosis Of Peripheral Blood Phagocytes In Patients With Multiple Sclerosis

Objective To explore the potential role of phagocytic function in the pathogenesis of multiple sclerosis(MS).Methods The patients with multiple sclerosis(MS)(n=15,4 males and 11 females,aged 42.73 +13.44)who were admitted to the Department of Neurology of the First Affiliated Hospital of Bengbu Medical College from March 2017 to March 2019 and who met the revised diagnostic criteria of Mc Donald Criteria MS and Lublin-Rheingold MS typing criteria,and the healthy persons without neurological and immune system diseases(n=15,5 males and 10 females)who underwent outpatient physical examination during the same period were collected.Peripheral venous blood samples and routine blood tests were performed in 15 patients with multiple sclerosis.The disability scores were evaluated by EDSS.The proportion of peripheral blood phagocytes(monocytes and neutrophils)in total white blood cells in blood routine of MS group and control group was counted.Flow cytometry was used to detect the phagocytosis of fluorescent microspheres by phagocytes in each group.The differences in the proportion of phagocytes and the phagocytosis rate between the two groups were analyzed and compared,and whether there was a linear correlation between the phagocytosis rate and clinical EDSS score.Results The proportion of peripheral blood mononuclear cells increased and the phagocytosis rate decreased in MS patients,with significant difference between the control group and the control group(P < 0.05);the proportion of peripheral blood neutrophils increased and the phagocytosis rate decreased in MS patients,with significant difference between the control group and the control group(P < 0.05);there was no correlation between the proportion of peripheral blood mononuclear cells and the phagocytosis rate in MS patients with different clinical EDSS scores(P >0.05).There was no correlation between the percentage of neutrophils and the phagocytosis rate in MS patients with different clinical EDSS scores(P > 0.05).Conclusion The proportion of peripheral blood monocytes and neutrophils increased in MS patients,but the phagocytosis rate of monocytes and neutrophils decreased,suggesting that phagocytes may be involved in the pathogenesis of MS.The abnormality of phagocyte proportion and phagocytosis rate may not be related to clinical severity.