Correlation Between Intestinal Flora Composition And Epilepsy

Objectives :Epilepsy is a common disease in central nervous system diseases,which is caused by abnormal discharge of neurons.According to the location and spread of abnormal discharge neurons,different dysfunctions can be caused,which can be manifested as different obstacles such as movement,sensation,consciousness,behavior and autonomic nervous system.According to the etiology of epilepsy,it can be divided into idiopathic epilepsy,cryptogenic epilepsy and symptomatic epilepsy.But the etiology of epilepsy is complex.The epilepsy that can not be identified in clinic accounts for 60%-70% of epilepsy patients.Recently,a large number of studies have shown that intestinal bacteria may affect the occurrence of central nervous system diseases through immune,metabolic,endocrine and other ways.Is there any correlation between intestinal microflora and epilepsy? The purpose of this study is to analyze the intestinal flora of three groups(normal person group,epilepsy group and refractory epilepsy group)by 16 S rRNA sequence design method.The relationship between intestinal flora disorder and epilepsy was analyzed by comparing the bacterial composition of the three groups.Meanwhile,intestinal flora and difficulty of seizure control was analyzed.Animal experiments were designed to further explore the possibility and mechanism of fecal bacteria transplantation in the treatment of epilepsy by constructing epilepsy mice model.Methods:From November 2017 to April 2018,22 patients with epilepsy and 13 patients with refractory epilepsy were randomly selected as the experimental group and 9 normal persons as the control group,aged from 18 to 60 years old.The intestinal microflora DNA was collected from feces and analyzed by Illuminate Hiseq PE250 16 SrDNA sequencing technology.Intestinal microflora were compared in biotaxonomy,Alpha diversity analysis,Beta diversity analysis and inter-group species diversity analysis.Results :1.Comparison of intestinal flora among normal subjects,epilepsy patients and refractory epilepsy patients.There were significant differences in intestinal flora among the three groups(P=0.008).Actinomycetes in normal group were significantly lower than those in epilepsy group and refractory epilepsy group.At the family level,Prevotellae in normal group was significantly higher than those in epilepsy group and refractory epilepsy group.Actinomycetes,protoplasts,alkaloids,daphne,breviscapaceae and sphingomycidae were significantly lower than those in epilepsy group and refractory epilepsy group.At the genus level,Prevotella in normal group was significantly higher than those in epilepsy group and refractory epilepsy group,while Bifidobacterium,fRuminococcaceae,Alcaligenes,Alistipes and Gemmiger in the actinomycetes group were significantly lower than those in the epilepsy group and refractory epilepsy group.2.Comparison of intestinal flora between epilepsy group and refractory epilepsy group.There were no significant differences in abundance or diversity of the whole intestinal flora between epilepsy group and refractory epilepsy group(P = 0.076),as well as the prevotellae or actinomycetes family.3.After transplanting fecal bacteria in healthy mice,the degree and frequency of seizures in mice in fecal transplantation group decreased significantly,compared with the tetraazo group(P <0.05).At the same time,the cognitive ability and memory ability of mice improved significantly.The levels of GABA,Gly,and Gulu in brain tissue changed significantly(P<0.05).MDA levels in mouse brain tissue were decreased and GSH levels were increased,with significant differences compared with the tetraazo groups,respectively(P<0.05).Conclusions:1.There was significant difference in intestinal flora between normal and epileptic patients.2.There was no significant difference in intestinal flora between epilepsy patients and refractory epilepsy patients.3.Faecal transplantation had the effect of inhibiting seizures in chronic epilepsy mice by improving the composition of intestinal bacteria.The mechanism was related to promoting the expression of antioxidant protein and reducing the structural damage of nerve cells after epilepsy.