Study On The Relationship Between Intestinal Flora And Anxiety And Depression Disorder Of Epilepsy And Epilepsy

ObjectivesThe purpose of this study is to investigate the composition and distribution of the microbiome in the stool samples of patients with epilepsy and epilepsy anxiety and depression comorbidities,to explore the differences in intestinal microflora between epilepsy patients and normal people,and changes in intestinal microflora and anxiety in epilepsy patients depression comorbidity and the interaction between different severity of attacks,provide a new strategy for clinical treatment of bacterial flora to slow down the disease.MethodsFrom November 2019 to January 2020,30 patients with temporal lobe epilepsy who were treated in neurology clinic of Xijing Hospital were included into epilepsy group in strict accordance with the inclusive criteria,and the epilepsy group was divided into RE?Refractory epilepsy?group and NRE?Non refractory epilepsy?group according to the ILAE diagnostic classification criteria;The patients were divided into NHS3-1?1-6 points?group?NHS3-2?7-12 points?group?NHS3-3?13-18 points?group according to NHS3 score results;The patients were divided into B1?epilepsy with no anxiety and depression comorbidity?group?B2?epilepsy with depression comorbidity?group?B3?epilepsy with anxiety and depression comorbidity?group according to HAMA,SAS and HAMD,SDS score results.During the same period,30 healthy family members of epilepsy patients were collected as a control group and general data were collected.Collect the stool samples of all subjects according to the requirements,and use the Illumina high-throughput sequencing platform to perform high-throughput sequencing and statistical analysis on the 16SrDNAV4-V5 area of all the stool samples of all subjects,and finally compare the epilepsy patients with normal people,epilepsy anxiety and depressiondifferences in intestinal flora between subgroups of comorbid patients and subgroups with different severity of seizures.Results?1?There was no significant difference in alpha and beta diversity in intestinal flora between CON group and RE Group,CON group and NRE group,RE group and NRE group,groups NHS3-1?NHS3-2 and NHS3-3,groups B1?B2 and B3?P>0.05?.?2?The results of metastats difference analysis between CON group and RE group showed that the abundance of intestinal flora in RE group was significantly different from that in CON group?P<0.05?: At the family level,the abundance of Propionibacterium,Microbacteriae and Stachyloccaceae increased,while that of Moraxellaceae and Planococcaceae decreased;At the genus level,the abundance of Propionibacterium,Johnsonella,Microcella,Staphylococus increased,while that of Acinetobacter,Dialister,Lysinibillus and Dielma decreased;At the species level,the abundance of Campylobacter_concisus,Microcellus_Alcaliphila,Ruminocaceae_Bacterium,Campylobacter_rreolyticus increased,while that of Coprocccus_comes,Bacteroides_plebeius,Eucharacterium_ramulus and Gut_metagenome decreased.?3?The results of Lefse analysis between CON group and RE group showed that the abundance of s_Bacterbides_plebeius and g_Megamonas in CON group and RE group had the greatest influence on the difference effect?P<0.05?.?4?The results of metastats showed that the abundance of intestinal flora in NRE group was significantly different from that in CON group?P<0.05?: The abundance of Proteobacteria in the phylum level was decreased;The abundance of Moraxellaceae,Planococcaceae,Neisseriae,Enterobacteriaee in the family level was decreased,and the abundance of Leptotrichiaceae,Eucharacteriaceae was increased.At the genus level,the abundance of Acinetobacter?Lysinibacillus?Eikenella?Intestinibacter?Rikenella?Candidatus_Soleaferrea ? Acidaminococcus decreased,while that of Shuttleworthia ?Sneathia?Mogibacterium?Murdochiella?Peptostreptococcus?Faecalitalea?Eubacterium increased.The abundance of Bacteroidetes_bacterium ? Intestinibacter_bartlettii ?Acidaminococcus_fermentans?Escherichia_coli in species level was decreased,while that of Collinsella_tanakaei?Eubacterium_sp._cL-10-1-3?Ruminococcus_sp._WAL₁7306?Campylobacter_hominis ? Mogibacterium_diversum ? Bacteroides_coagulans ?Eubacterium_limosum increased.?5?The results of Lefse analysis between CON group and NRE group showed that the abundance of g_Alloprevotella and g_Megamonas in CON group and NRE group had the greatest influence on the difference effect?P<0.05?.?6?The results of metastats analysis showed that the abundance of intestinal flora in RE group was different from that in NRE group?P<0.05?: The abundance of Tenerictes and Synergistes in the phylum level in RE group was significantly lower than that in NRE group.The abundance of Staphylococacceae,Protonibacteriae and Microbacteriae in the family level in RE group was higher than that in NRE group.The abundance of Dialister?Pseudobutyrivibrio?Mitsuokella?Pyramidobacter?Senegalimassilia?Cloacibacillus?Ruminococcus?Klebsiella in genus level in RE group was significantly decreased,while that of Staphylococcus ? Propionibacterium ? Stomatobaculum ? Cloacibacillus ?Microcella increased.At the species level,the abundance of Coprococcus_comes significantly decreased,while tha of Campylobacter_concisus and Microcella_alkaliphila increased.?7?The results of Lefse analysis between RE Group and NRE group showed that the abundance of s_Prevotella_stercorea and p_Bacteroidetes in RE Group and NRE group had the greatest influence on the difference effect?P<0.05?.?8?The results of metastats difference analysis among the NHS3₁,NHS3₂,and NHS3₃ groups showed that the abundance of intestinal flora among the NHS3 subgroups was significantly different at the family,genus,and species levels: Compared with the NHS3₁group,the abundance of Eubacteriaceae and Eubacterium in the intenstinal flora of NHS3₂ and NHS3₃ groups decreased progressively,while the abundance of Cloacibacillus increased progressively?P<0.05?.?9?The results of Lefse analysis between NHS3₁ and NHS3₂ and NHS3₃ groups showed that the abundance of s_uncultured_Clostridiates_bacterium and g_Coprococcus in NHS3₂ group and NHS3₃group had the greatest influence on the difference effect?P<0.05?.?10?The results of metastats analysis showed that there were significant differences in the levels of phylum,family,genus and species among B1,B2 and B3 groups?P<0.05?: Compared with B1 group,the abundance of Proteobacteria in the phylum level,the abundance of Pasteurellaceae,Ruminocaceae,Alcaligenaceae in the family level in B2 group were higher than those in B1 group.At the genus level,the abundance ofRuminococcus and Faecalibacterium in B2 group was decreased,while the abundance of Haemophilus was increased.Compared with group B1,the abundance of Campylobacteraceae in group B3 was higher than that in group B1.The abundance of Billophila,Cloacibacillus,Erysipelatocostricium,Integinibacter,Parasutterella,Pepsostreptococcus,Campylobacter and Johnsonella in genus level in group B3 was higher.Compared with group B2,the abundance of Acidaminoccaceae in family level in group B3 was increased,at the genus level,the abundance of Phascolarctobacterium ?Ruminococcus?Desulfovibrio?Cloacibacillus?Adlercreutzia increased,while Sutterella was decreased.?11?The results of Lefse analysis among B1,B2 and B3 groups showed that g_Klebsell,s_Megasphaera_elsdenii and s_Bacteroides_stercoris were the most influential factors on the difference effect?P<0.05?.Conclusions?1?There are differences in intestinal flora abundance between patients with epilepsy and normal people,patients with refractory epilepsy and non refractory epilepsy at different taxonomic levels,but there is no difference in species diversity.?2?The intestinal flora of patients with intractable and non intractable epilepsy was most affected by the abundance of g_Megamonas.?3?With the increase in the severity of seizures,the abundance of Eubacteriaceae in the intenstinail flora and the abundance of Eubacterium at the genus level decreased.However,the abundance of Cloacibacillus at the family level incerased.?4?There are differences in the diversity of intestinal flora among patients with different types of epilepsy anxiety and depression comorbidity at different taxonomic levels,but there is no difference in species diversity.