Correlation Between Plasma Peroxiredoxin 1 And Homocysteine Levels With The Incidence Of Cerebral Infarction

Object: To investigate the relationship between peroxireoxin I(PRDX1),Homocysteine(Hcy)with cerebral infarction,the concentration of PRDX1 and Hcy in plasma of patients with cerebral infarction was detected.And to analyse the correlation of the PRDX1、Hcy and associated risk factors,National Institute of Health stroke scale(NIHSS)was scored.To evaluate whether plasma PRDX1 can be used as biochemical indicators for early diagnosis of cerebral infarction,the relationship between the level of Hcy and the incidence of cerebral infarction was analyzed.Finally,it would provide experimental basis for early clinical diagnosis and treatment of acute cerebral infarction.Methods: 113 cases of patients with cerebral infarction were collected who were admitted to the neurology ward in our hospital from May 2017 to May 2018.At the same time,the 63 person who healthy-check-up in our hospital physical examination center were set as control.The cerebral infarction group was confirmed to be the first cerebral infarction by Magnetic Resonance Imaging(MRI),and all were admitted within 72 hours of onset.The general information of patients were recorded,including gender,age,smoking history,drinking history,hypertension,coronary heart disease,diabetes history,etc.Then,stroke severity was assessed on admission using the NIHSS.According to the NIHSS score,the patients were divided into two groups:patients with mild stroke(NIHSS score ≦5)and patients with moderate to severe stroke(NIHSS score>5).The blood sample was sent to our hospital’s laboratory on the day of physical examination or on the second day in the early morning.All subjects were taken to detect the Hcy,low density lipoprotein(LDL-C),total cholesterol(TC),triglycerides(TG),high density lipoprotein(HDL-C),fasting glucose and glycated hemoglobin.The serum levels of PRDX1 were detected by enzyme-linked immunosorbent(ELISA).The levels of PRDX1 and Hcy were compared between the case group and control group.All data were processed by SPSS 23 statistical software.Results:(1)There was a statistically significant difference in age,gender,hypertension,diabetes mellitus,smoking history,high-density lipoprotein and triglycerides between the normal control group and the cerebral infarction group(P <0.05).(2)Compared with the normal control group the level of serum PRDX1 was significant higher than the cerebral infarction group,and the difference is statistically significant(P <0.05).The level of serum Hcy in the normal group was lower than the cerebral infarction group,and the difference is statistically significant(P <0.05).(3)The level of serum PRDX1 in patients with lower NIHSS(NIHSS≦5)was(62.53±27.18)ng/ml,while the level in patients with high NIHSS score(NIHSS>5)was(62.5±27.18)ng/ml,showing that the difference was not statistically significant(P=0.348>0.05).The concentration of serum Hcy in patients with lower NIHSS(NIHSS≦5)was(16.59±11.22)umol/L,while the concentration in patients with high NIHSS score(NIHSS>5)was(19.50±9.31)umol/L,implying that the difference was not statistically significant(P =0.160>0.05).(4)In the cerebral infarction group,multivariate logistic regression analysis showed that Hcy,PRDX1,HDL-C,age and smoking were closely related to the occurrence of cerebral infarction(P<0.05).The Hcy(OR,1.146;95%CI,1.046-1.256;P=0.004),smoking(OR,5.113;95%CI,1.595-16.389;P=0.006),age(OR,1.046;95%CI,1.009-1.084;P=0.014)are independent risk factors for cerebral infarction.While HDL-C(OR,0.057;95% CI,0.008-0.338;P=0.003)、PRDX1(OR,0.961;95% CI,0.944-0.979;P=0.000)are independent protective factors for cerebral infarction.Conclusion:(1)Compared with the normal control group the level of serum PRDX I was significant higher than the cerebral infarction group,and its level was not related to the NIHSS score.(2)The level of serum Hcy in the normal group was lower than the cerebral infarction group,and is the independent risk factor for cerebral infarction.Among the patients with cerebral infarction,the patients with mild increase in Hcy accounted for the majority,but the level of Hcy was irrelevant to the severity of the disease.(3)The gender,age,hypertension,diabetes mellitus,smoking history and TG are the risk factors for cerebral infarction.Moreover,the smoking is an independent protective factor.